Detailed information of TA system TA035903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3137053..3137713
• Replicon: chromosome
• Accession: NZ_AP022519
• Organism: Enterobacter cloacae strain STN0717-73

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLF40_RS15270
• Protein ID: WP_033145931.1
• Coordinates: 3137360..3137713 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: JLF40_RS15265
• Protein ID: WP_029741460.1
• Coordinates: 3137053..3137355 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF40_RS15240	3132962..3134323	+	1362	WP_003036316.1	hypothetical protein	-
JLF40_RS15245	3134644..3134976	-	333	WP_033145927.1	type II toxin-antitoxin system PemK/MazF family toxin	-
JLF40_RS15250	3134977..3135234	-	258	WP_033145928.1	antitoxin	-
JLF40_RS15255	3135526..3135915	-	390	WP_033145929.1	RidA family protein	-
JLF40_RS15260	3136057..3136977	+	921	WP_033145930.1	LysR family transcriptional regulator	-
JLF40_RS15265	3137053..3137355	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
JLF40_RS15270	3137360..3137713	-	354	WP_033145931.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF40_RS15275	3137891..3138784	-	894	WP_033145932.1	LysR family transcriptional regulator	-
JLF40_RS15280	3138955..3139563	+	609	WP_080277780.1	glutathione S-transferase family protein	-
JLF40_RS15285	3139607..3140557	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
JLF40_RS15290	3140654..3141571	-	918	WP_033145933.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13577.40 Da
• Isoelectric Point: 8.0361

>T35903 WP_033145931.1 NZ_AP022519:c3137713-3137360 [Enterobacter cloacae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
MLCAGNKVGNERRFYDEMLLVADQEFTHWLDRLKERE

Nucleotide

• Length: 354 bp

>T35903 NZ_AP022519:c3137713-3137360 [Enterobacter cloacae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGTACTGGCATC
ATGCTTTGTGCTGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCAGGAATTCAC
TCACTGGCTGGATAGATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT35903 WP_029741460.1 NZ_AP022519:c3137355-3137053 [Enterobacter cloacae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT35903 NZ_AP022519:c3137355-3137053 [Enterobacter cloacae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGTGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGACGGCTCGCACTATGAGTTTGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79