Detailed information of TA system TA035844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3581300..3581957
• Replicon: chromosome
• Accession: NZ_AP022508
• Organism: Enterobacter bugandensis strain STN0717-56

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLD64_RS16875
• Protein ID: WP_048957972.1
• Coordinates: 3581300..3581599 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLD64_RS16880
• Protein ID: WP_047367527.1
• Coordinates: 3581610..3581957 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLD64_RS16865	3577621..3579816	+	2196	WP_028014148.1	type I secretion system permease/ATPase	-
JLD64_RS16870	3579827..3580996	+	1170	WP_088204896.1	HlyD family efflux transporter periplasmic adaptor subunit	-
JLD64_RS16875	3581300..3581599	+	300	WP_048957972.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLD64_RS16880	3581610..3581957	+	348	WP_047367527.1	HigA family addiction module antidote protein	Antitoxin
JLD64_RS16885	3581959..3582690	-	732	WP_193031123.1	helix-turn-helix transcriptional regulator	-
JLD64_RS16890	3582788..3583207	+	420	WP_023619020.1	nuclear transport factor 2 family protein	-
JLD64_RS16895	3583253..3584434	-	1182	WP_199886685.1	PLP-dependent aminotransferase family protein	-
JLD64_RS16900	3584455..3585342	-	888	WP_045260881.1	LysR family transcriptional regulator	-
JLD64_RS16905	3585440..3586042	+	603	WP_028014155.1	short chain dehydrogenase	-
JLD64_RS16910	3586036..3586770	-	735	WP_199886686.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	3563537..3633267	69730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-102)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11538.15 Da
• Isoelectric Point: 10.0104

>T35844 WP_048957972.1 NZ_AP022508:3581300-3581599 [Enterobacter bugandensis]
MINHFRDQWLKDFFLYGKSSNVIPSSLESALARKLDMINAATSHRELRSPPGNMYESLNPPLAGYSAIRVNRQYRLVFRW
SEGKADDLYLSPHKYTPYK

Nucleotide

• Length: 300 bp

>T35844 NZ_AP022508:3581300-3581599 [Enterobacter bugandensis]
ATGATTAATCATTTTCGTGATCAATGGCTTAAGGATTTTTTTCTGTACGGGAAATCCAGCAATGTGATCCCTTCAAGCCT
TGAATCAGCCTTAGCGAGGAAGCTGGATATGATCAACGCTGCCACCTCGCACCGGGAGCTGAGATCCCCGCCAGGCAATA
TGTATGAATCGCTGAATCCCCCGCTTGCAGGGTACTCTGCGATTCGCGTTAACAGGCAATACAGGTTGGTATTCAGGTGG
TCAGAGGGTAAAGCAGACGATCTCTACCTCTCTCCGCACAAGTACACGCCATACAAATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13126.08 Da
• Isoelectric Point: 7.0141

>AT35844 WP_047367527.1 NZ_AP022508:3581610-3581957 [Enterobacter bugandensis]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIRLAKAFDTTIEFWLNLQLNVD
VWEAQTNSRTQEELSRIKTIAEIMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT35844 NZ_AP022508:3581610-3581957 [Enterobacter bugandensis]
ATGACTCTTCAACAGGCTCTACGCAAACCCACTACGCCGGGCGATGTGTTGCAGTATGAATATCTTGAGCCGCTCAATCT
GAAAATTAGCGATCTCGCGGAAATGCTTAACGTGCACCGTAACACCATCAGCGCTCTCGTTAATAATAATCGTAAACTGA
CGGCCGATATGGCAATCAGACTAGCCAAGGCGTTTGATACCACTATTGAATTCTGGTTGAACCTGCAGCTAAACGTCGAC
GTCTGGGAAGCGCAAACCAATTCCCGCACTCAGGAGGAGTTGAGCCGTATAAAAACGATTGCAGAAATCATGGCAAAAAG
GAAATCAGGCCAGCCGGATGTGGCCTGA