Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1190468..1191117 Replicon chromosome
Accession NZ_AP022498
Organism Enterobacter kobei strain STN0717-53

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0PLQ3
Locus tag JLH17_RS05625 Protein ID WP_014882864.1
Coordinates 1190755..1191117 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag JLH17_RS05620 Protein ID WP_014882863.1
Coordinates 1190468..1190767 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLH17_RS05610 1186893..1188866 + 1974 WP_045269817.1 PhoX family phosphatase -
JLH17_RS05615 1188969..1190357 + 1389 WP_045269818.1 phenylalanine transporter -
JLH17_RS05620 1190468..1190767 - 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
JLH17_RS05625 1190755..1191117 - 363 WP_014882864.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLH17_RS05630 1191323..1192558 + 1236 WP_023332295.1 MFS transporter -
JLH17_RS05635 1192574..1193599 + 1026 WP_045269819.1 sugar phosphate isomerase/epimerase -
JLH17_RS05640 1193592..1194734 + 1143 WP_045269820.1 Gfo/Idh/MocA family oxidoreductase -
JLH17_RS05645 1194810..1195781 + 972 WP_045269821.1 LacI family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13952.92 Da        Isoelectric Point: 6.7114

>T35818 WP_014882864.1 NZ_AP022498:c1191117-1190755 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGNKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T35818 NZ_AP022498:c1191117-1190755 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAAACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT35818 WP_014882863.1 NZ_AP022498:c1190767-1190468 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT35818 NZ_AP022498:c1190767-1190468 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PLQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References