Detailed information of TA system TA035814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31816..32546
• Replicon: chromosome
• Accession: NZ_AP022498
• Organism: Enterobacter kobei strain STN0717-53

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7I7DTA0
• Locus tag: JLH17_RS00155
• Protein ID: WP_045269322.1
• Coordinates: 31816..32130 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLH17_RS00160
• Protein ID: WP_023331687.1
• Coordinates: 32127..32546 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH17_RS00130	27481..28569	+	1089	WP_032635252.1	cellulase family glycosylhydrolase	-
JLH17_RS00135	28472..29656	-	1185	WP_045269345.1	multidrug efflux MFS transporter EmrD	-
JLH17_RS00140	29832..30665	-	834	WP_045269321.1	DMT family transporter	-
JLH17_RS00145	30728..31174	-	447	WP_014881927.1	GNAT family N-acetyltransferase	-
JLH17_RS00150	31239..31328	-	90	WP_023331685.1	type I toxin-antitoxin system toxin TisB	-
JLH17_RS00155	31816..32130	+	315	WP_045269322.1	type II toxin-antitoxin system HigB family toxin	Toxin
JLH17_RS00160	32127..32546	+	420	WP_023331687.1	helix-turn-helix domain-containing protein	Antitoxin
JLH17_RS00165	32696..32794	+	99	WP_023331688.1	ilvB operon leader peptide IvbL	-
JLH17_RS00170	32901..34589	+	1689	WP_014881930.1	acetolactate synthase large subunit	-
JLH17_RS00175	34593..34880	+	288	WP_010426528.1	acetolactate synthase small subunit	-
JLH17_RS00180	34963..35556	+	594	WP_014881931.1	transcriptional regulator UhpA	-
JLH17_RS00185	35553..37058	+	1506	WP_045134486.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12459.62 Da
• Isoelectric Point: 10.2825

>T35814 WP_045269322.1 NZ_AP022498:31816-32130 [Enterobacter kobei]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALMFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T35814 NZ_AP022498:31816-32130 [Enterobacter kobei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTGATCCAGTTTCCGCAACACAGAGAGGAGCTTCTCTTTCTGGG
GCGAACCATCGAAAAGAGCCATTGCACGACGCCTGTTGCGCTAAGAAAAATCTTTCCAACGCTAGATAATTTGAAGTATC
TCGATAAGCATTATGTTATTGATATTGCTAACAACAACCTCAGGGTAGTGGCTCTCATGTTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15561.18 Da
• Isoelectric Point: 5.6761

>AT35814 WP_023331687.1 NZ_AP022498:32127-32546 [Enterobacter kobei]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT35814 NZ_AP022498:32127-32546 [Enterobacter kobei]
ATGATGATCGTAGCCGATGCAATGAAAGCAACGCATGCCCTTGTCGCGGCGGTTCCTTTATTGGGTGAACATCCAAACGA
GAAAGATTACCAGGACGCACTTGAGCTGGTTGAGTATTTACTGATGAACGAACCTGGCAGCCCTTTGCTGGATATCGTAT
GTGCAAGAATTCGTCGTTACGAAGCCAATCGACCCGAAATTGTCGCCTTACGCCAGGAGATGGAATCTGTCCCAGTGGGC
ATTGCCGTTTTAAGAACCCTTATGGATCAATACAAACTAACTATTTCGGATTTTCAGGATGAAATTGGCAGTAAATCAAT
GGTTTCAAGGGTGTTGAACGGTCAACGTCAGCTGACGTTGAACCATATAAAAAAACTGGCTGCCCGCTTTGGGGTATCGC
CTGCGCTGTTTATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A7I7DTA0