Detailed information of TA system TA035812

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 197151..197808
• Replicon: plasmid pSTN0717-36-1
• Accession: NZ_AP022495
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JLF47_RS24850
• Protein ID: WP_000270043.1
• Coordinates: 197151..197501 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLF47_RS24855
• Protein ID: WP_000124640.1
• Coordinates: 197506..197808 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS24815	193625..194122	-	498	WP_000062185.1	hypothetical protein	-
JLF47_RS24820	194125..194613	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
JLF47_RS24825	194710..195045	+	336	WP_000683477.1	hypothetical protein	-
JLF47_RS24830	195060..195530	-	471	WP_001281821.1	hypothetical protein	-
JLF47_RS24835	195523..195894	-	372	WP_000516918.1	hypothetical protein	-
JLF47_RS24840	195905..196099	-	195	WP_000343597.1	hypothetical protein	-
JLF47_RS24845	196440..196988	-	549	WP_001061573.1	hypothetical protein	-
JLF47_RS24850	197151..197501	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF47_RS24855	197506..197808	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JLF47_RS24860	197835..198128	-	294	WP_001239998.1	hypothetical protein	-
JLF47_RS24865	198217..198489	-	273	WP_001043046.1	HU family DNA-binding protein	-
JLF47_RS24870	198547..199074	-	528	WP_004201083.1	thermonuclease family protein	-
JLF47_RS24875	199077..199382	-	306	WP_171820680.1	hypothetical protein	-
JLF47_RS24880	199294..200151	-	858	WP_001167036.1	hypothetical protein	-
JLF47_RS24885	200138..200368	-	231	WP_000972665.1	hypothetical protein	-
JLF47_RS24890	200368..200886	-	519	WP_000210757.1	nitrite reductase	-
JLF47_RS24895	200883..201329	-	447	WP_000919343.1	hypothetical protein	-
JLF47_RS24900	201329..201688	-	360	WP_000422769.1	hypothetical protein	-
JLF47_RS24905	201746..202174	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / aph(6)-Id / aph(3'')-Ib / aadA2 / blaGES-4	-	1..210204	210204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T35812 WP_000270043.1 NZ_AP022495:197151-197501 [Citrobacter portucalensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T35812 NZ_AP022495:197151-197501 [Citrobacter portucalensis]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT35812 WP_000124640.1 NZ_AP022495:197506-197808 [Citrobacter portucalensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT35812 NZ_AP022495:197506-197808 [Citrobacter portucalensis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9