Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 36539..37078 Replicon plasmid pSTN0717-27-3
Accession NZ_AP022489
Organism Citrobacter portucalensis strain STN0717-27

Toxin (Protein)


Gene name yafQ Uniprot ID J2DZM3
Locus tag JLF36_RS25465 Protein ID WP_004150484.1
Coordinates 36539..36814 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A5H6MLU0
Locus tag JLF36_RS25470 Protein ID WP_001570852.1
Coordinates 36818..37078 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF36_RS25415 32021..32215 - 195 WP_200926052.1 hypothetical protein -
JLF36_RS25420 32232..32771 - 540 WP_079969741.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
JLF36_RS25425 32787..33344 - 558 WP_200926053.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
JLF36_RS25430 33347..33658 - 312 WP_200926054.1 hypothetical protein -
JLF36_RS25435 33794..34087 - 294 WP_200926055.1 hypothetical protein -
JLF36_RS25440 34084..34326 - 243 WP_200926056.1 hypothetical protein -
JLF36_RS25445 34346..34645 - 300 WP_200926057.1 hypothetical protein -
JLF36_RS25450 34623..34850 - 228 WP_200926058.1 hypothetical protein -
JLF36_RS25455 34861..35388 - 528 WP_059347886.1 ankyrin repeat domain-containing protein -
JLF36_RS25460 35720..36385 + 666 WP_200926059.1 hypothetical protein -
JLF36_RS25465 36539..36814 - 276 WP_004150484.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
JLF36_RS25470 36818..37078 - 261 WP_001570852.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
JLF36_RS25475 37328..37585 - 258 WP_032445815.1 hypothetical protein -
JLF36_RS25480 39004..39261 + 258 WP_032445815.1 hypothetical protein -
JLF36_RS25485 39438..40022 - 585 WP_200926060.1 hypothetical protein -
JLF36_RS25490 40048..40560 - 513 WP_200926061.1 hypothetical protein -
JLF36_RS25495 40667..40969 - 303 WP_004148936.1 hypothetical protein -
JLF36_RS25500 41037..41735 - 699 WP_050511162.1 N-6 DNA methylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..43729 43729


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-83)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10750.40 Da        Isoelectric Point: 9.6586

>T35793 WP_004150484.1 NZ_AP022489:c36814-36539 [Citrobacter portucalensis]
MTTRTIEYSGQFQKDVKKAQKRHKDMSKLKALMTLLINDALPLPQVYKDHPLQGNYKGYRDAHIEPDWLLIYKITDEVLR
FERTGTHSDLF

Download         Length: 276 bp

>T35793 NZ_AP022489:c36814-36539 [Citrobacter portucalensis]
ATGACCACAAGGACGATTGAATACTCAGGCCAGTTCCAGAAAGATGTGAAGAAGGCTCAGAAGCGTCATAAAGACATGAG
CAAGCTTAAAGCCCTTATGACGCTGTTAATCAATGATGCCTTGCCATTGCCTCAAGTCTACAAAGATCACCCTCTACAGG
GGAACTACAAAGGCTATAGGGATGCTCACATAGAACCAGACTGGCTACTCATCTACAAGATAACTGATGAAGTCCTACGC
TTTGAGCGAACCGGAACACATTCAGACCTGTTTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9239.63 Da        Isoelectric Point: 5.0336

>AT35793 WP_001570852.1 NZ_AP022489:c37078-36818 [Citrobacter portucalensis]
MAANAFVRARIDETLKNEAAVVLAGMGLTVSDLVRITLTKVAKEKALPFDMRVPNELTANTIANSEKGVDVHTAKDADDL
FNQLGI

Download         Length: 261 bp

>AT35793 NZ_AP022489:c37078-36818 [Citrobacter portucalensis]
ATGGCCGCAAATGCATTTGTTCGTGCTCGTATAGATGAAACACTGAAAAATGAAGCTGCAGTAGTGCTTGCCGGAATGGG
CTTAACCGTTTCCGATCTGGTTCGTATCACTCTGACTAAGGTAGCCAAAGAAAAGGCTCTGCCGTTCGATATGCGTGTTC
CTAACGAGCTTACTGCTAATACCATCGCTAACAGTGAGAAAGGCGTAGACGTTCATACAGCTAAAGATGCTGATGATCTG
TTTAATCAGCTTGGTATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J2DZM3

Antitoxin

Source ID Structure
AlphaFold DB A0A5H6MLU0

References