Detailed information of TA system TA035770

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55966..56220
• Replicon: plasmid pSTN0717-20-1
• Accession: NZ_AP022483
• Organism: Escherichia coli strain STN0717-20

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: JLE48_RS23050
• Protein ID: WP_015387399.1
• Coordinates: 56014..56220 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	55966..56027 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLE48_RS23015	51618..51830	+	213	WP_005012601.1	hypothetical protein	-
JLE48_RS23020	52131..52220	-	90	Protein_64	IS1 family transposase	-
JLE48_RS23025	52275..52952	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
JLE48_RS23030	52952..53299	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
JLE48_RS23035	53319..54890	+	1572	WP_023149734.1	IS66 family transposase	-
JLE48_RS23040	54928..55544	-	617	Protein_68	IS1 family transposase	-
JLE48_RS23045	55645..55827	+	183	WP_000968309.1	hypothetical protein	-
-	55966..56027	-	62	NuclAT_1	-	Antitoxin
-	55966..56027	-	62	NuclAT_1	-	Antitoxin
-	55966..56027	-	62	NuclAT_1	-	Antitoxin
-	55966..56027	-	62	NuclAT_1	-	Antitoxin
JLE48_RS23050	56014..56220	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLE48_RS23055	56504..56761	+	258	WP_000083833.1	replication regulatory protein RepA	-
JLE48_RS23060	56997..57071	+	75	WP_032336874.1	RepA leader peptide Tap	-
JLE48_RS23065	57064..57510	+	447	Protein_73	incFII family plasmid replication initiator RepA	-
JLE48_RS23070	57456..57938	-	483	Protein_74	VENN motif pre-toxin domain-containing protein	-
JLE48_RS23075	58831..60051	+	1221	WP_000410951.1	arginine deiminase	-
JLE48_RS23080	60062..60973	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaCTX-M-55 / qnrS1 / aac(3)-IId / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..157329	157329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T35770 WP_015387399.1 NZ_AP022483:56014-56220 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T35770 NZ_AP022483:56014-56220 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT35770 NZ_AP022483:c56027-55966 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin