Detailed information of TA system TA035766

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 15883..16153
• Replicon: plasmid pSTN0717-20-1
• Accession: NZ_AP022483
• Organism: Escherichia coli strain STN0717-20

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLE48_RS22820
• Protein ID: WP_001312861.1
• Coordinates: 15995..16153 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	15883..15946 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLE48_RS22775	11994..12559	+	566	Protein_15	class I SAM-dependent methyltransferase	-
JLE48_RS22780	12585..12803	+	219	WP_001496291.1	hypothetical protein	-
JLE48_RS22785	13213..13419	+	207	WP_000275856.1	hypothetical protein	-
JLE48_RS22790	13445..13984	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
JLE48_RS22795	14052..14285	+	234	WP_000005990.1	DUF905 family protein	-
JLE48_RS22800	14313..14510	+	198	Protein_20	hypothetical protein	-
JLE48_RS22805	14565..14999	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
JLE48_RS22810	14996..15715	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
JLE48_RS22815	15727..15915	-	189	WP_001299721.1	hypothetical protein	-
-	15727..15951	+	225	NuclAT_0	-	-
-	15727..15951	+	225	NuclAT_0	-	-
-	15727..15951	+	225	NuclAT_0	-	-
-	15727..15951	+	225	NuclAT_0	-	-
-	15883..15946	-	64	-	-	Antitoxin
JLE48_RS22820	15995..16153	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLE48_RS22825	17074..17361	+	288	WP_000107535.1	hypothetical protein	-
JLE48_RS22830	17479..18300	+	822	WP_001234426.1	DUF945 domain-containing protein	-
JLE48_RS22835	18597..19199	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
JLE48_RS22840	19520..19903	+	384	WP_001151524.1	relaxosome protein TraM	-
JLE48_RS22845	20090..20779	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaCTX-M-55 / qnrS1 / aac(3)-IId / sitABCD	iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..157329	157329

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35766 WP_001312861.1 NZ_AP022483:15995-16153 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35766 NZ_AP022483:15995-16153 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT35766 NZ_AP022483:c15946-15883 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin