Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3710027..3710568 Replicon chromosome
Accession NZ_AP022482
Organism Escherichia coli strain STN0717-20

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag JLE48_RS17810 Protein ID WP_000615976.1
Coordinates 3710290..3710568 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag JLE48_RS17805 Protein ID WP_000729704.1
Coordinates 3710027..3710287 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE48_RS17780 3705810..3706595 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
JLE48_RS17785 3706567..3708279 + 1713 Protein_3483 flagellar biosynthesis protein FlhA -
JLE48_RS17790 3708384..3708662 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
JLE48_RS17795 3708655..3709011 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
JLE48_RS17800 3709068..3709841 - 774 WP_000543895.1 C40 family peptidase -
JLE48_RS17805 3710027..3710287 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
JLE48_RS17810 3710290..3710568 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
JLE48_RS17815 3710724..3711464 + 741 WP_001225679.1 murein L,D-transpeptidase -
JLE48_RS17820 3711435..3712202 - 768 WP_000333380.1 class II glutamine amidotransferase -
JLE48_RS17825 3712408..3712986 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3708655..3718859 10204


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T35761 WP_000615976.1 NZ_AP022482:3710290-3710568 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T35761 NZ_AP022482:3710290-3710568 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT35761 WP_000729704.1 NZ_AP022482:3710027-3710287 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT35761 NZ_AP022482:3710027-3710287 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References