Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1785877..1786379 Replicon chromosome
Accession NZ_AP022482
Organism Escherichia coli strain STN0717-20

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JLE48_RS08445 Protein ID WP_000767829.1
Coordinates 1786125..1786379 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JLE48_RS08440 Protein ID WP_001259255.1
Coordinates 1785877..1786128 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE48_RS08415 1781054..1782121 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JLE48_RS08420 1782121..1783191 - 1071 WP_094315765.1 histidinol-phosphate transaminase -
JLE48_RS08425 1783188..1784492 - 1305 WP_094315766.1 histidinol dehydrogenase -
JLE48_RS08430 1784498..1785397 - 900 WP_094315767.1 ATP phosphoribosyltransferase -
JLE48_RS08435 1785543..1785593 - 51 WP_001364200.1 his operon leader peptide -
JLE48_RS08440 1785877..1786128 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JLE48_RS08445 1786125..1786379 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JLE48_RS08450 1786462..1787286 + 825 WP_000754737.1 SDR family oxidoreductase -
JLE48_RS08455 1787332..1788261 + 930 WP_000803366.1 LysR family transcriptional regulator -
JLE48_RS08460 1788476..1788538 + 63 WP_010723108.1 membrane protein YoeI -
JLE48_RS08465 1788528..1789886 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JLE48_RS08470 1790065..1791123 + 1059 WP_000492321.1 transport protein YeeE -
JLE48_RS08475 1791137..1791364 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T35746 WP_000767829.1 NZ_AP022482:1786125-1786379 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T35746 NZ_AP022482:1786125-1786379 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT35746 WP_001259255.1 NZ_AP022482:1785877-1786128 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT35746 NZ_AP022482:1785877-1786128 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References