Detailed information of TA system TA035731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3976825..3977519
• Replicon: chromosome
• Accession: NZ_AP022478
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: V0YKG6
• Locus tag: JLF30_RS18985
• Protein ID: WP_001263485.1
• Coordinates: 3976825..3977223 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: JLF30_RS18990
• Protein ID: WP_000554758.1
• Coordinates: 3977226..3977519 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS18955	3971994..3973238	-	1245	WP_001586695.1	esterase FrsA	-
-	3972654..3972734	-	81	NuclAT_12	-	-
-	3972654..3972734	-	81	NuclAT_12	-	-
-	3972654..3972734	-	81	NuclAT_12	-	-
-	3972654..3972734	-	81	NuclAT_12	-	-
JLF30_RS18960	3973330..3973788	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JLF30_RS18965	3974049..3975506	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
JLF30_RS18970	3975563..3975915	-	353	Protein_3707	peptide chain release factor H	-
JLF30_RS18975	3975911..3976117	-	207	Protein_3708	RtcB family protein	-
JLF30_RS18980	3976363..3976815	-	453	WP_001059899.1	GNAT family N-acetyltransferase	-
JLF30_RS18985	3976825..3977223	-	399	WP_001263485.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JLF30_RS18990	3977226..3977519	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JLF30_RS18995	3977571..3978626	-	1056	WP_001226177.1	DNA polymerase IV	-
JLF30_RS19000	3978697..3979482	-	786	WP_000207554.1	putative lateral flagellar export/assembly protein LafU	-
JLF30_RS19005	3979454..3981166	+	1713	Protein_3714	flagellar biosynthesis protein FlhA	-
JLF30_RS19010	3981706..3982203	-	498	WP_000006263.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15418.84 Da
• Isoelectric Point: 7.3840

>T35731 WP_001263485.1 NZ_AP022478:c3977223-3976825 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLCQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T35731 NZ_AP022478:c3977223-3976825 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGTGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT35731 WP_000554758.1 NZ_AP022478:c3977519-3977226 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT35731 NZ_AP022478:c3977519-3977226 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: V0YKG6

Antitoxin

• AlphaFold DB: S1QAE3