35684

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4130203..4130930 Replicon chromosome
Accession NZ_AP022452
Organism Enterobacter kobei strain STW0522-62

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag JLF39_RS19895 Protein ID WP_000558568.1
Coordinates 4130619..4130930 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag JLF39_RS19890 Protein ID WP_000990392.1
Coordinates 4130203..4130622 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF39_RS19860 4125890..4126252 - 363 WP_000783215.1 arsenite efflux transporter metallochaperone ArsD -
JLF39_RS19865 4126300..4126653 - 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
JLF39_RS19870 4126969..4127562 + 594 WP_044133241.1 hypothetical protein -
JLF39_RS19875 4127986..4128645 + 660 WP_000078540.1 hypothetical protein -
JLF39_RS19880 4128642..4128971 + 330 WP_000121260.1 hypothetical protein -
JLF39_RS19885 4128964..4130166 + 1203 Protein_3895 phage tail protein -
JLF39_RS19890 4130203..4130622 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
JLF39_RS19895 4130619..4130930 - 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
JLF39_RS19900 4131141..4131521 + 381 Protein_3898 gluconate permease -
JLF39_RS19905 4131571..4132266 - 696 WP_000370412.1 lactate utilization protein C -
JLF39_RS19910 4132259..4133686 - 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
JLF39_RS19915 4133697..4134416 - 720 WP_001102107.1 (Fe-S)-binding protein -
JLF39_RS19920 4134491..4134814 - 324 Protein_3902 D-lactate dehydrogenase -
JLF39_RS19925 4134829..4135526 - 698 Protein_3903 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4081134..4130930 49796
- flank IS/Tn - - 4134829..4135206 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T35684 WP_000558568.1 NZ_AP022452:c4130930-4130619 [Enterobacter kobei]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T35684 NZ_AP022452:c4130930-4130619 [Enterobacter kobei]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT35684 WP_000990392.1 NZ_AP022452:c4130622-4130203 [Enterobacter kobei]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT35684 NZ_AP022452:c4130622-4130203 [Enterobacter kobei]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGAACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References