Detailed information of TA system TA035674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1258075..1258724
• Replicon: chromosome
• Accession: NZ_AP022452
• Organism: Enterobacter kobei strain STW0522-62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLF39_RS05995
• Protein ID: WP_023332294.1
• Coordinates: 1258362..1258724 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2W0GCS7
• Locus tag: JLF39_RS05990
• Protein ID: WP_014882863.1
• Coordinates: 1258075..1258374 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF39_RS05980	1254500..1256473	+	1974	WP_200924935.1	PhoX family phosphatase	-
JLF39_RS05985	1256576..1257964	+	1389	WP_200924936.1	phenylalanine transporter	-
JLF39_RS05990	1258075..1258374	-	300	WP_014882863.1	helix-turn-helix transcriptional regulator	Antitoxin
JLF39_RS05995	1258362..1258724	-	363	WP_023332294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF39_RS06000	1258930..1260165	+	1236	WP_023332295.1	MFS transporter	-
JLF39_RS06005	1260181..1261206	+	1026	WP_200924937.1	sugar phosphate isomerase/epimerase	-
JLF39_RS06010	1261199..1262341	+	1143	WP_200924938.1	Gfo/Idh/MocA family oxidoreductase	-
JLF39_RS06015	1262417..1263388	+	972	WP_200924939.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13953.90 Da
• Isoelectric Point: 6.2260

>T35674 WP_023332294.1 NZ_AP022452:c1258724-1258362 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T35674 NZ_AP022452:c1258724-1258362 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTGAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAGACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10860.61 Da
• Isoelectric Point: 9.8627

>AT35674 WP_014882863.1 NZ_AP022452:c1258374-1258075 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT35674 NZ_AP022452:c1258374-1258075 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0GCS7