Detailed information of TA system TA035667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4911882..4912484
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLF31_RS23995
• Protein ID: WP_058690340.1
• Coordinates: 4912173..4912484 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B2XUJ3
• Locus tag: JLF31_RS23990
• Protein ID: WP_032635182.1
• Coordinates: 4911882..4912172 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS23975	4909380..4910282	+	903	WP_014833793.1	formate dehydrogenase subunit beta	-
JLF31_RS23980	4910279..4910914	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
JLF31_RS23985	4910911..4911840	+	930	WP_014885738.1	formate dehydrogenase accessory protein FdhE	-
JLF31_RS23990	4911882..4912172	-	291	WP_032635182.1	helix-turn-helix domain-containing protein	Antitoxin
JLF31_RS23995	4912173..4912484	-	312	WP_058690340.1	hypothetical protein	Toxin
JLF31_RS24000	4912713..4913633	+	921	WP_058690339.1	alpha/beta hydrolase	-
JLF31_RS24005	4913642..4914583	-	942	WP_023336483.1	fatty acid biosynthesis protein FabY	-
JLF31_RS24010	4914628..4915065	-	438	WP_014885747.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF31_RS24015	4915062..4915943	-	882	WP_047343713.1	virulence factor BrkB family protein	-
JLF31_RS24020	4915937..4916536	-	600	WP_200923749.1	glucose-1-phosphatase	-
JLF31_RS24025	4916656..4917456	-	801	WP_047343714.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12264.32 Da
• Isoelectric Point: 10.1230

>T35667 WP_058690340.1 NZ_AP022446:c4912484-4912173 [Enterobacter kobei]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLVTQPNYGDLIQNTGGLRKIRWFSGSKGKRGGVRVIYFHRTREFEIRLLLIY
RKGIKDDLSAKEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T35667 NZ_AP022446:c4912484-4912173 [Enterobacter kobei]
ATGCTATTCATTGAAACGGATATTTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTTCAGGT
TTTCCTGGTAACGCAACCTAATTATGGCGATCTGATTCAGAATACGGGGGGCCTGCGAAAGATTCGCTGGTTTTCTGGAA
GCAAGGGAAAGCGTGGTGGAGTAAGGGTTATTTATTTTCATCGTACGCGTGAGTTTGAAATTAGGTTGCTGCTCATTTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAAGGAGAAAGCCATTCTGAAAAAGATGATAGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10703.22 Da
• Isoelectric Point: 10.5888

>AT35667 WP_032635182.1 NZ_AP022446:c4912172-4911882 [Enterobacter kobei]
MDKALFERLTQSIAQMNEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Nucleotide

• Length: 291 bp

>AT35667 NZ_AP022446:c4912172-4911882 [Enterobacter kobei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATGAACGAAATTGCCGAGGGCCAACGCGAGCCGTC
GCGTACGTTTCAAATCGATGCGATGAAAATAAAAGAAATTCGGCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTCAGTGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCCACAGGCCCGGCCAAAGCGCTCTTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCTGCGCTATCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XUJ3