Detailed information of TA system TA035655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1254909..1255558
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLF31_RS06170
• Protein ID: WP_023332294.1
• Coordinates: 1255196..1255558 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2W0GCS7
• Locus tag: JLF31_RS06165
• Protein ID: WP_014882863.1
• Coordinates: 1254909..1255208 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS06155	1251334..1253307	+	1974	WP_078310105.1	PhoX family phosphatase	-
JLF31_RS06160	1253410..1254798	+	1389	WP_058690612.1	phenylalanine transporter	-
JLF31_RS06165	1254909..1255208	-	300	WP_014882863.1	helix-turn-helix transcriptional regulator	Antitoxin
JLF31_RS06170	1255196..1255558	-	363	WP_023332294.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF31_RS06175	1255764..1256999	+	1236	WP_023332295.1	MFS transporter	-
JLF31_RS06180	1257015..1258040	+	1026	WP_032637265.1	sugar phosphate isomerase/epimerase	-
JLF31_RS06185	1258033..1259175	+	1143	WP_058690611.1	Gfo/Idh/MocA family oxidoreductase	-
JLF31_RS06190	1259251..1260273	+	1023	WP_058690610.1	LacI family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13953.90 Da
• Isoelectric Point: 6.2260

>T35655 WP_023332294.1 NZ_AP022446:c1255558-1255196 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T35655 NZ_AP022446:c1255558-1255196 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAGACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10860.61 Da
• Isoelectric Point: 9.8627

>AT35655 WP_014882863.1 NZ_AP022446:c1255208-1254909 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT35655 NZ_AP022446:c1255208-1254909 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGTGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Structures

Antitoxin

• AlphaFold DB: A0A2W0GCS7