Detailed information of TA system TA035651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 62743..63389
• Replicon: chromosome
• Accession: NZ_AP022446
• Organism: Enterobacter kobei strain STW0522-60

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLF31_RS00335
• Protein ID: WP_058690226.1
• Coordinates: 63039..63389 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLF31_RS00330
• Protein ID: WP_032635284.1
• Coordinates: 62743..63042 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF31_RS00300	58157..59116	-	960	WP_023336511.1	DUF523 and DUF1722 domain-containing protein	-
JLF31_RS00305	59219..59629	-	411	WP_014881954.1	hydroxyisourate hydrolase	-
JLF31_RS00310	59729..60454	-	726	WP_200923754.1	MerR family transcriptional regulator	-
JLF31_RS00315	60572..61096	+	525	WP_014881956.1	lipocalin family protein	-
JLF31_RS00320	61175..62173	+	999	WP_058690220.1	class I SAM-dependent methyltransferase	-
JLF31_RS00325	62276..62713	+	438	WP_058690221.1	acetyltransferase	-
JLF31_RS00330	62743..63042	-	300	WP_032635284.1	XRE family transcriptional regulator	Antitoxin
JLF31_RS00335	63039..63389	-	351	WP_058690226.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF31_RS00340	63920..64261	-	342	WP_058690222.1	SymE family type I addiction module toxin	-
JLF31_RS00345	64390..64551	+	162	WP_072205328.1	DUF3950 domain-containing protein	-
JLF31_RS00350	64797..66302	+	1506	WP_058690223.1	DNA polymerase	-
JLF31_RS00355	66438..66758	-	321	WP_023305176.1	TA system toxin CbtA family protein	-
JLF31_RS00360	66792..67157	-	366	WP_032660963.1	type IV toxin-antitoxin system YeeU family antitoxin	-
JLF31_RS00365	67189..67410	-	222	WP_023305178.1	DUF987 family protein	-
JLF31_RS00370	67419..67889	-	471	WP_023305179.1	DNA repair protein RadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13481.52 Da
• Isoelectric Point: 6.9819

>T35651 WP_058690226.1 NZ_AP022446:c63389-63039 [Enterobacter kobei]
MWTVLFGKVFELWLLEQEEGLQEKVLADMLNLQNFGPRLPRPYADTVKGSQYNNMKELRIQYAGRPIRALFAFDPERQAI
VLCAGNKSNDKAFYEKLIRIADDEFTRHLTSLETAK

Nucleotide

• Length: 351 bp

>T35651 NZ_AP022446:c63389-63039 [Enterobacter kobei]
ATGTGGACGGTACTGTTCGGAAAGGTGTTTGAGCTATGGTTGCTTGAACAGGAAGAAGGGTTACAGGAAAAGGTGCTGGC
AGACATGCTAAATCTACAAAACTTTGGACCTCGTCTTCCTCGTCCCTATGCCGATACTGTAAAAGGCTCGCAATATAACA
ATATGAAAGAGTTACGTATTCAATATGCTGGCCGTCCGATTCGTGCTTTATTCGCATTTGACCCCGAACGTCAGGCGATT
GTGCTTTGCGCAGGCAACAAGAGTAACGACAAGGCATTTTATGAAAAACTCATTCGCATTGCAGATGATGAATTTACCCG
GCATCTGACAAGCCTGGAGACAGCCAAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11160.04 Da
• Isoelectric Point: 6.9842

>AT35651 WP_032635284.1 NZ_AP022446:c63042-62743 [Enterobacter kobei]
VKTLNELIASRSPESQQRIKEMAEEMILETGLQLLREELRLSQKNVAELMGVSQPAVTQIEQRGNELKIATLKRYIEAMG
GKLCIDVELPTGKRVAFHI

Nucleotide

• Length: 300 bp

>AT35651 NZ_AP022446:c63042-62743 [Enterobacter kobei]
GTGAAAACCCTAAATGAATTAATTGCATCACGCTCACCGGAAAGCCAACAACGAATTAAAGAAATGGCAGAGGAAATGAT
CCTTGAAACGGGTCTGCAACTTTTACGTGAAGAGCTCAGACTTTCGCAAAAAAATGTTGCCGAGCTAATGGGGGTGAGCC
AGCCCGCAGTAACACAAATAGAGCAACGTGGAAATGAACTAAAAATTGCCACGCTTAAGCGCTACATTGAGGCAATGGGT
GGGAAACTTTGTATTGACGTTGAACTTCCAACAGGAAAACGCGTTGCGTTTCATATCTAA