Detailed information of TA system TA035648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4613430..4614032
• Replicon: chromosome
• Accession: NZ_AP022431
• Organism: Enterobacter kobei strain STW0522-51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JLF35_RS22020
• Protein ID: WP_200923298.1
• Coordinates: 4613721..4614032 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8B2XUJ3
• Locus tag: JLF35_RS22015
• Protein ID: WP_032635182.1
• Coordinates: 4613430..4613720 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF35_RS22000	4610928..4611830	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
JLF35_RS22005	4611827..4612462	+	636	WP_008501832.1	formate dehydrogenase cytochrome b556 subunit	-
JLF35_RS22010	4612459..4613388	+	930	WP_047343709.1	formate dehydrogenase accessory protein FdhE	-
JLF35_RS22015	4613430..4613720	-	291	WP_032635182.1	helix-turn-helix domain-containing protein	Antitoxin
JLF35_RS22020	4613721..4614032	-	312	WP_200923298.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF35_RS22025	4614261..4615181	+	921	WP_047624625.1	alpha/beta hydrolase	-
JLF35_RS22030	4615190..4616131	-	942	WP_023336483.1	fatty acid biosynthesis protein FabY	-
JLF35_RS22035	4616176..4616613	-	438	WP_014885747.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF35_RS22040	4616610..4617491	-	882	WP_014885748.1	virulence factor BrkB family protein	-
JLF35_RS22045	4617485..4618084	-	600	WP_045268693.1	glucose-1-phosphatase	-
JLF35_RS22050	4618204..4619004	-	801	WP_014885750.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12245.33 Da
• Isoelectric Point: 10.2130

>T35648 WP_200923298.1 NZ_AP022431:c4614032-4613721 [Enterobacter kobei]
MLFIETDIFTEDVKTLLDDDEYHKLQVFLVMQPNNGDLIQNTGGLRKIRWFSGSKGKRGGVRVIYFHRTREFEIRLLLIY
RKGIKDDLSAKEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T35648 NZ_AP022431:c4614032-4613721 [Enterobacter kobei]
ATGCTATTCATTGAAACGGATATTTTCACAGAAGACGTCAAAACGTTACTGGATGACGACGAATACCATAAGCTTCAGGT
TTTCCTGGTAATGCAACCTAATAATGGCGATCTGATTCAGAATACGGGGGGCCTGCGAAAGATTCGCTGGTTTTCTGGAA
GCAAGGGAAAGCGTGGTGGAGTAAGGGTTATTTATTTTCATCGTACGCGTGAGTTTGAAATTAGGTTGCTGCTCATTTAT
CGCAAAGGGATCAAAGATGACCTGTCCGCGAAGGAGAAAGCCATTCTGAAAAAGATGATAGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10703.22 Da
• Isoelectric Point: 10.5888

>AT35648 WP_032635182.1 NZ_AP022431:c4613720-4613430 [Enterobacter kobei]
MDKALFERLTQSIAQMNEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Nucleotide

• Length: 291 bp

>AT35648 NZ_AP022431:c4613720-4613430 [Enterobacter kobei]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATGAACGAAATTGCCGAGGGCCAACGCGAGCCGTC
GCGTACGTTTCAAATCGATGCGATGAAAATAAAAGAAATTCGCCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTCAGTGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCCACAGGCCCGGCCAAAGCGCTCTTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCTGCGCTATCGCAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B2XUJ3