Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1176485..1177134 Replicon chromosome
Accession NZ_AP022431
Organism Enterobacter kobei strain STW0522-51

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0PLQ3
Locus tag JLF35_RS05580 Protein ID WP_014882864.1
Coordinates 1176772..1177134 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2W0GCS7
Locus tag JLF35_RS05575 Protein ID WP_014882863.1
Coordinates 1176485..1176784 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF35_RS05565 1172910..1174883 + 1974 WP_193970167.1 PhoX family phosphatase -
JLF35_RS05570 1174986..1176374 + 1389 WP_014882862.1 phenylalanine transporter -
JLF35_RS05575 1176485..1176784 - 300 WP_014882863.1 helix-turn-helix transcriptional regulator Antitoxin
JLF35_RS05580 1176772..1177134 - 363 WP_014882864.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF35_RS05585 1177340..1178575 + 1236 WP_193970166.1 MFS transporter -
JLF35_RS05590 1178591..1179616 + 1026 WP_193970165.1 sugar phosphate isomerase/epimerase -
JLF35_RS05595 1179609..1180751 + 1143 WP_200923373.1 Gfo/Idh/MocA family oxidoreductase -
JLF35_RS05600 1180827..1181798 + 972 WP_014882868.1 LacI family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-80)


Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13952.92 Da        Isoelectric Point: 6.7114

>T35637 WP_014882864.1 NZ_AP022431:c1177134-1176772 [Enterobacter kobei]
MWNVETTDRFDQWYFEQTTALKEDVLAMMHILAEFGPTLGRPYVDTVKASAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGNKTGCNQKRFYNEMITLADVEYSRHLADKEAIWQR

Download         Length: 363 bp

>T35637 NZ_AP022431:c1177134-1176772 [Enterobacter kobei]
ATGTGGAATGTCGAGACAACGGACAGATTTGATCAATGGTACTTCGAACAAACAACAGCATTAAAAGAAGATGTGCTAGC
GATGATGCATATTCTTGCTGAGTTTGGCCCAACGCTTGGGCGGCCATACGTAGATACCGTGAAGGCTTCAGCCTATGCGA
ACATGAAGGAATTACGGATTCAACATGCGGGAAATCCCATCAGAGCTTTTTTTGCTTTTGATCCTGACCGTAAGGCAATT
GTGCTCTGTGCTGGAAACAAAACCGGTTGCAATCAAAAACGGTTTTATAACGAAATGATTACGCTGGCTGACGTTGAGTA
CAGCAGACATCTGGCGGATAAGGAGGCCATATGGCAACGCTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10860.61 Da        Isoelectric Point: 9.8627

>AT35637 WP_014882863.1 NZ_AP022431:c1176784-1176485 [Enterobacter kobei]
MATLKELMAQQSAESQERIAAKVDVIRRIVALNQLREELNISQTELAAAMGVKQPTVAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Download         Length: 300 bp

>AT35637 NZ_AP022431:c1176784-1176485 [Enterobacter kobei]
ATGGCAACGCTAAAAGAACTCATGGCACAACAGAGCGCGGAAAGTCAGGAGCGTATTGCGGCTAAAGTTGATGTAATACG
CAGAATTGTTGCCCTGAACCAACTGCGGGAAGAGCTCAATATTTCGCAGACAGAACTGGCCGCGGCAATGGGGGTAAAAC
AACCAACTGTGGCAAAAATCGAACAGCCGGGAAACGATCCCCGGCTTTCAACACTCAAACGCTATGTCAGTGCGCTCGGC
GGTGAAATGAGCATTGATGTGACGTTACCGAATGGGAAACGAATAGCGTTTCAGATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0PLQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A2W0GCS7

References