Detailed information of TA system TA035615

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32755..33176
• Replicon: plasmid pSTW0522-31-1
• Accession: NZ_AP022410
• Organism: Escherichia coli strain STW0522-31

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A168PPB2
• Locus tag: JLE49_RS22985
• Protein ID: WP_012881135.1
• Coordinates: 33015..33176 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	32755..32962 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLE49_RS22955	28458..28979	+	522	WP_012881138.1	single-stranded DNA-binding protein	-
JLE49_RS22960	29035..29268	+	234	WP_000005971.1	DUF905 domain-containing protein	-
JLE49_RS22965	29332..31290	+	1959	WP_012881137.1	ParB/RepB/Spo0J family partition protein	-
JLE49_RS22970	31345..31779	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
JLE49_RS22975	31776..32495	+	720	WP_012881136.1	plasmid SOS inhibition protein A	-
JLE49_RS22980	32492..32806	+	315	WP_000872086.1	hypothetical protein	-
-	32755..32962	+	208	NuclAT_0	-	Antitoxin
-	32755..32962	+	208	NuclAT_0	-	Antitoxin
-	32755..32962	+	208	NuclAT_0	-	Antitoxin
-	32755..32962	+	208	NuclAT_0	-	Antitoxin
JLE49_RS22985	33015..33176	+	162	WP_012881135.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLE49_RS22990	33414..33683	-	270	WP_001388591.1	hypothetical protein	-
JLE49_RS22995	34022..34345	+	324	WP_000533253.1	hypothetical protein	-
JLE49_RS23000	34404..34646	+	243	WP_000540588.1	hypothetical protein	-
JLE49_RS23005	35643..35939	+	297	WP_001272251.1	hypothetical protein	-
JLE49_RS23010	36049..36870	+	822	WP_001234437.1	DUF945 domain-containing protein	-
JLE49_RS23015	37166..37768	-	603	WP_000243715.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaCTX-M-14 / blaTEM-1B	-	1..82247	82247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 6000.17 Da
• Isoelectric Point: 8.5137

>T35615 WP_012881135.1 NZ_AP022410:33015-33176 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTHLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T35615 NZ_AP022410:33015-33176 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACACCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 208 bp

>AT35615 NZ_AP022410:32755-32962 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A168PPB2

Antitoxin