Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3415909..3416588 Replicon chromosome
Accession NZ_AP022409
Organism Escherichia coli strain STW0522-31

Toxin (Protein)


Gene name higB Uniprot ID U9XN95
Locus tag JLE49_RS16260 Protein ID WP_000057540.1
Coordinates 3416286..3416588 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag JLE49_RS16255 Protein ID WP_000806442.1
Coordinates 3415909..3416250 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE49_RS16245 3412153..3413085 - 933 WP_023155692.1 glutaminase A -
JLE49_RS16250 3413347..3415851 + 2505 WP_023155691.1 copper-exporting P-type ATPase CopA -
JLE49_RS16255 3415909..3416250 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
JLE49_RS16260 3416286..3416588 - 303 WP_000057540.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLE49_RS16265 3416721..3417515 + 795 WP_000365180.1 TraB/GumN family protein -
JLE49_RS16270 3417719..3418198 + 480 WP_000186631.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
JLE49_RS16275 3418235..3419887 - 1653 WP_000771768.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
JLE49_RS16280 3420105..3421325 + 1221 WP_001251608.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-100)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11781.35 Da        Isoelectric Point: 10.2638

>T35607 WP_000057540.1 NZ_AP022409:c3416588-3416286 [Escherichia coli]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTSLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T35607 NZ_AP022409:c3416588-3416286 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCTCGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCCGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT35607 WP_000806442.1 NZ_AP022409:c3416250-3415909 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT35607 NZ_AP022409:c3416250-3415909 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XN95

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References