Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1864584..1865086 Replicon chromosome
Accession NZ_AP022409
Organism Escherichia coli strain STW0522-31

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JLE49_RS08795 Protein ID WP_000767829.1
Coordinates 1864832..1865086 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JLE49_RS08790 Protein ID WP_001259255.1
Coordinates 1864584..1864835 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE49_RS08765 1859761..1860828 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JLE49_RS08770 1860828..1861898 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
JLE49_RS08775 1861895..1863199 - 1305 WP_001317939.1 histidinol dehydrogenase -
JLE49_RS08780 1863205..1864104 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JLE49_RS08785 1864250..1864300 - 51 WP_001364200.1 his operon leader peptide -
JLE49_RS08790 1864584..1864835 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JLE49_RS08795 1864832..1865086 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JLE49_RS08800 1865169..1865993 + 825 WP_200922276.1 SDR family oxidoreductase -
JLE49_RS08805 1866039..1866968 + 930 WP_000803351.1 LysR family transcriptional regulator -
JLE49_RS08810 1867183..1867245 + 63 WP_010723108.1 membrane protein YoeI -
JLE49_RS08815 1867235..1868593 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JLE49_RS08820 1868772..1869830 + 1059 WP_000492346.1 transport protein YeeE -
JLE49_RS08825 1869844..1870071 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T35598 WP_000767829.1 NZ_AP022409:1864832-1865086 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T35598 NZ_AP022409:1864832-1865086 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT35598 WP_001259255.1 NZ_AP022409:1864584-1864835 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT35598 NZ_AP022409:1864584-1864835 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References