Detailed information of TA system TA035592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 11563..12220
• Replicon: plasmid pSTW0522-30-1
• Accession: NZ_AP022400
• Organism: Citrobacter portucalensis strain STW0522-30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JLF43_RS23895
• Protein ID: WP_000270043.1
• Coordinates: 11870..12220 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLF43_RS23890
• Protein ID: WP_000124640.1
• Coordinates: 11563..11865 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF43_RS23840	7197..7625	+	429	WP_000591076.1	hypothetical protein	-
JLF43_RS23845	7683..8042	+	360	WP_000422769.1	hypothetical protein	-
JLF43_RS23850	8042..8488	+	447	WP_000919343.1	hypothetical protein	-
JLF43_RS23855	8485..9003	+	519	WP_000210757.1	nitrite reductase	-
JLF43_RS23860	9003..9233	+	231	WP_000972665.1	hypothetical protein	-
JLF43_RS23865	9220..10077	+	858	WP_001167036.1	hypothetical protein	-
JLF43_RS23870	9989..10294	+	306	WP_171820680.1	hypothetical protein	-
JLF43_RS23875	10297..10824	+	528	WP_004201083.1	thermonuclease family protein	-
JLF43_RS23880	10882..11154	+	273	WP_001043046.1	HU family DNA-binding protein	-
JLF43_RS23885	11243..11536	+	294	WP_001239998.1	hypothetical protein	-
JLF43_RS23890	11563..11865	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JLF43_RS23895	11870..12220	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF43_RS23900	12383..12931	+	549	WP_001061573.1	hypothetical protein	-
JLF43_RS23905	13272..13466	+	195	WP_000343597.1	hypothetical protein	-
JLF43_RS23910	13477..13848	+	372	WP_000516918.1	hypothetical protein	-
JLF43_RS23915	13841..14311	+	471	WP_001281821.1	hypothetical protein	-
JLF43_RS23920	14326..14661	-	336	WP_000683477.1	hypothetical protein	-
JLF43_RS23925	14758..15246	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
JLF43_RS23930	15249..15746	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaGES-4 / aadA2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aph(3')-Ia	-	1..211224	211224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T35592 WP_000270043.1 NZ_AP022400:c12220-11870 [Citrobacter portucalensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T35592 NZ_AP022400:c12220-11870 [Citrobacter portucalensis]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT35592 WP_000124640.1 NZ_AP022400:c11865-11563 [Citrobacter portucalensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT35592 NZ_AP022400:c11865-11563 [Citrobacter portucalensis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9