Detailed information of TA system TA035572

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 198182..198839
• Replicon: plasmid pSTW0522-27-1
• Accession: NZ_AP022395
• Organism: Citrobacter portucalensis strain STW0522-27

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: JLF33_RS25300
• Protein ID: WP_000270043.1
• Coordinates: 198182..198532 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLF33_RS25305
• Protein ID: WP_000124640.1
• Coordinates: 198537..198839 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF33_RS25265	194656..195153	-	498	WP_000062185.1	hypothetical protein	-
JLF33_RS25270	195156..195644	-	489	WP_001273096.1	DUF1643 domain-containing protein	-
JLF33_RS25275	195741..196076	+	336	WP_000683477.1	hypothetical protein	-
JLF33_RS25280	196091..196561	-	471	WP_001281821.1	hypothetical protein	-
JLF33_RS25285	196554..196925	-	372	WP_000516918.1	hypothetical protein	-
JLF33_RS25290	196936..197130	-	195	WP_000343597.1	hypothetical protein	-
JLF33_RS25295	197471..198019	-	549	WP_001061573.1	hypothetical protein	-
JLF33_RS25300	198182..198532	+	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF33_RS25305	198537..198839	+	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
JLF33_RS25310	198866..199159	-	294	WP_001239998.1	hypothetical protein	-
JLF33_RS25315	199248..199520	-	273	WP_001043046.1	HU family DNA-binding protein	-
JLF33_RS25320	199578..200105	-	528	WP_004201083.1	thermonuclease family protein	-
JLF33_RS25325	200108..200413	-	306	WP_171820680.1	hypothetical protein	-
JLF33_RS25330	200325..201182	-	858	WP_001167036.1	hypothetical protein	-
JLF33_RS25335	201169..201399	-	231	WP_000972665.1	hypothetical protein	-
JLF33_RS25340	201399..201917	-	519	WP_000210757.1	nitrite reductase	-
JLF33_RS25345	201914..202360	-	447	WP_000919343.1	hypothetical protein	-
JLF33_RS25350	202360..202719	-	360	WP_000422769.1	hypothetical protein	-
JLF33_RS25355	202777..203205	-	429	WP_000591076.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A) / aph(6)-Id / aph(3'')-Ib / aadA2 / blaGES-4	-	1..211235	211235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T35572 WP_000270043.1 NZ_AP022395:198182-198532 [Citrobacter portucalensis]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T35572 NZ_AP022395:198182-198532 [Citrobacter portucalensis]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT35572 WP_000124640.1 NZ_AP022395:198537-198839 [Citrobacter portucalensis]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT35572 NZ_AP022395:198537-198839 [Citrobacter portucalensis]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9