Detailed information of TA system TA035517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 14615..15272
• Replicon: plasmid pBML2537
• Accession: NZ_AP022377
• Organism: Providencia stuartii strain BML2537

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: G7Y71_RS20645
• Protein ID: WP_000270043.1
• Coordinates: 14922..15272 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G7Y71_RS20640
• Protein ID: WP_000124640.1
• Coordinates: 14615..14917 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Y71_RS20590	9720..10082	+	363	WP_021293829.1	hypothetical protein	-
G7Y71_RS20595	10075..11073	+	999	WP_164457030.1	hypothetical protein	-
G7Y71_RS20600	11083..11529	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
G7Y71_RS20605	11526..12044	+	519	WP_164457031.1	nitrite reductase	-
G7Y71_RS20610	12044..12274	+	231	WP_000972664.1	hypothetical protein	-
G7Y71_RS20615	12261..13118	+	858	WP_164457032.1	hypothetical protein	-
G7Y71_RS20625	13349..13876	+	528	WP_011872875.1	thermonuclease family protein	-
G7Y71_RS20630	13934..14206	+	273	WP_001043047.1	HU family DNA-binding protein	-
G7Y71_RS20635	14295..14588	+	294	WP_001239997.1	chromosome segregation protein ParM	-
G7Y71_RS20640	14615..14917	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
G7Y71_RS20645	14922..15272	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G7Y71_RS20650	15435..15983	+	549	WP_001061574.1	transcriptional regulator	-
G7Y71_RS20660	16324..16518	+	195	WP_000343597.1	hypothetical protein	-
G7Y71_RS20665	16529..16900	+	372	WP_164457033.1	hypothetical protein	-
G7Y71_RS20670	16893..17363	+	471	WP_001281821.1	hypothetical protein	-
G7Y71_RS20675	17378..17713	-	336	WP_164457034.1	hypothetical protein	-
G7Y71_RS20680	17810..18298	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
G7Y71_RS20685	18301..18798	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / qacE / aac(6')-Iae / blaIMP-10	-	1..152754	152754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T35517 WP_000270043.1 NZ_AP022377:c15272-14922 [Providencia stuartii]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T35517 NZ_AP022377:c15272-14922 [Providencia stuartii]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT35517 WP_000124640.1 NZ_AP022377:c14917-14615 [Providencia stuartii]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT35517 NZ_AP022377:c14917-14615 [Providencia stuartii]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9