Detailed information of TA system TA035516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 62202..62794
• Replicon: plasmid pBML2531
• Accession: NZ_AP022376
• Organism: Providencia rettgeri strain BML2531

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q7A225
• Locus tag: G7Y73_RS22165
• Protein ID: WP_011039853.1
• Coordinates: 62202..62480 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: L7Z874
• Locus tag: G7Y73_RS22170
• Protein ID: WP_011039854.1
• Coordinates: 62480..62794 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G7Y73_RS22135	58009..58917	+	909	WP_011039846.1	hypothetical protein	-
G7Y73_RS22140	59012..59218	+	207	WP_011039847.1	hypothetical protein	-
G7Y73_RS22145	59203..59532	+	330	WP_011039848.1	hypothetical protein	-
G7Y73_RS22150	59529..59981	+	453	WP_109910770.1	hypothetical protein	-
G7Y73_RS22155	61022..61264	+	243	WP_011039851.1	hypothetical protein	-
G7Y73_RS22160	61708..62031	+	324	WP_109910769.1	hypothetical protein	-
G7Y73_RS22165	62202..62480	+	279	WP_011039853.1	type II toxin-antitoxin system toxin endoribonuclease HigB	Toxin
G7Y73_RS22170	62480..62794	+	315	WP_011039854.1	type II toxin antitoxin system addiction module antidote protein HigA	Antitoxin
G7Y73_RS22175	63053..63421	+	369	WP_109910768.1	hypothetical protein	-
G7Y73_RS22180	63621..64541	+	921	WP_011039857.1	hypothetical protein	-
G7Y73_RS22185	64573..64749	+	177	WP_011039858.1	hypothetical protein	-
G7Y73_RS22190	64775..65212	+	438	WP_109910766.1	plasmid protein	-
G7Y73_RS22195	65230..65952	+	723	WP_109910765.1	hypothetical protein	-
G7Y73_RS22200	66232..66747	+	516	WP_109910764.1	hypothetical protein	-
G7Y73_RS22205	66926..67120	-	195	WP_162598852.1	hypothetical protein	-
G7Y73_RS22210	67190..67369	+	180	WP_162598851.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / qacE / aac(6')-Il / blaOXA-2 / blaIMP-11	mrkB	1..84930	84930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10742.22 Da
• Isoelectric Point: 8.4392

>T35516 WP_011039853.1 NZ_AP022376:62202-62480 [Providencia rettgeri]
MIKSFKHKGLKLLFEKGVTSGVPAQDVDRINDRLQAIDTATEIGELNRQIYKLHPLKGDREGYWSITVRANWRITFQFIN
GDAYILNYEDYH

Nucleotide

• Length: 279 bp

>T35516 NZ_AP022376:62202-62480 [Providencia rettgeri]
ATGATAAAATCTTTCAAGCACAAAGGATTGAAACTGCTTTTTGAAAAGGGTGTTACTTCTGGTGTGCCTGCGCAAGATGT
AGACAGAATCAATGACCGTTTGCAGGCCATCGATACAGCGACAGAGATTGGTGAACTAAACCGCCAGATTTACAAATTAC
ATCCATTAAAGGGGGATCGGGAAGGTTACTGGTCTATCACTGTCCGGGCGAATTGGCGCATTACTTTTCAGTTCATTAAC
GGTGATGCTTACATTTTAAATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11536.99 Da
• Isoelectric Point: 6.7241

>AT35516 WP_011039854.1 NZ_AP022376:62480-62794 [Providencia rettgeri]
MRQFKVSHPGEMIARDLEDMGVSGRRFAHNIGVTPATVSRLLAGKTALTPSLSIRIAAALGSTPEFWLRLQSNYDLRQLE
NQIDTSGIVLYGESNEQQQNAQEH

Nucleotide

• Length: 315 bp

>AT35516 NZ_AP022376:62480-62794 [Providencia rettgeri]
ATGAGACAATTCAAGGTTTCACATCCGGGTGAGATGATAGCCCGCGATTTAGAGGATATGGGAGTGAGTGGTCGTCGTTT
TGCTCACAATATTGGTGTTACACCAGCAACCGTATCCCGTCTACTTGCGGGGAAAACTGCGTTGACCCCTTCTCTTTCGA
TTCGTATTGCTGCGGCACTGGGGAGTACGCCTGAGTTTTGGTTACGGCTACAGAGCAATTATGATCTGCGCCAGTTAGAA
AACCAAATCGATACATCCGGGATCGTCTTGTACGGTGAGTCGAACGAACAGCAGCAGAACGCGCAAGAGCATTAA

Structures

Toxin

• PDB: 4MCT
• PDB: 4YPB
• PDB: 4MCX
• PDB: 4YY3
• PDB: 4PX8
• PDB: 4ZSN
• PDB: 4W4G
• PDB: 5IWH
• PDB: 5IXL
• PDB: 4YZV

Antitoxin

• PDB: 4MCT
• PDB: 4MCX
• PDB: 6CHV
• PDB: 6CF1
• AlphaFold DB: A0A379FY56