Detailed information of TA system TA035470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 3099708..3100210
• Replicon: chromosome
• Accession: NZ_AP022362
• Organism: Escherichia coli strain E302

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: G6M96_RS15540
• Protein ID: WP_000767819.1
• Coordinates: 3099708..3099962 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: G6M96_RS15545
• Protein ID: WP_001259253.1
• Coordinates: 3099959..3100210 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6M96_RS15515	3095526..3095984	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
G6M96_RS15520	3096201..3097559	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
G6M96_RS15525	3097549..3097611	-	63	WP_010723108.1	membrane protein YoeI	-
G6M96_RS15530	3097826..3098755	-	930	WP_000803366.1	LysR family transcriptional regulator	-
G6M96_RS15535	3098801..3099625	-	825	WP_000754737.1	SDR family oxidoreductase	-
G6M96_RS15540	3099708..3099962	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
G6M96_RS15545	3099959..3100210	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
G6M96_RS15550	3100493..3100543	+	51	WP_001364200.1	his operon leader peptide	-
G6M96_RS15555	3100689..3101588	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
G6M96_RS15560	3101594..3102898	+	1305	WP_001296211.1	histidinol dehydrogenase	-
G6M96_RS15565	3102895..3103965	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
G6M96_RS15570	3103965..3105032	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3095526..3095984	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T35470 WP_000767819.1 NZ_AP022362:c3099962-3099708 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T35470 NZ_AP022362:c3099962-3099708 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT35470 WP_001259253.1 NZ_AP022362:c3100210-3099959 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT35470 NZ_AP022362:c3100210-3099959 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3