Detailed information of TA system TA035409

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sib/-
• Location: 295478..295720
• Replicon: chromosome
• Accession: NZ_AP022351
• Organism: Escherichia coli strain E138

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GR930_RS01455
• Protein ID: WP_001312861.1
• Coordinates: 295478..295636 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sib
Locus tag	-
Coordinates	295680..295720 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GR930_RS01420	290590..290817	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
GR930_RS01425	290905..291582	-	678	WP_001348626.1	PAS domain-containing protein	-
GR930_RS01430	291716..292099	-	384	WP_001151566.1	relaxosome protein TraM	-
GR930_RS01435	292430..293032	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
GR930_RS01440	293329..294150	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GR930_RS01445	294269..294556	-	288	WP_000107535.1	hypothetical protein	-
GR930_RS23245	294581..294787	-	207	WP_000275859.1	hypothetical protein	-
GR930_RS01455	295478..295636	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	295680..295720	-	41	NuclAT_1	-	Antitoxin
-	295680..295720	-	41	NuclAT_1	-	Antitoxin
-	295680..295720	-	41	NuclAT_1	-	Antitoxin
-	295680..295720	-	41	NuclAT_1	-	Antitoxin
GR930_RS01460	295747..297117	-	1371	Protein_273	IS3-like element IS150 family transposase	-
-	297165..297351	-	187	NuclAT_0	-	-
-	297165..297351	-	187	NuclAT_0	-	-
-	297165..297351	-	187	NuclAT_0	-	-
-	297165..297351	-	187	NuclAT_0	-	-
GR930_RS01465	297363..298082	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
GR930_RS01470	298079..298513	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
GR930_RS01475	298568..300526	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	tufA	292523..339364	46841

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35409 WP_001312861.1 NZ_AP022351:c295636-295478 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35409 NZ_AP022351:c295636-295478 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT35409 NZ_AP022351:c295720-295680 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin