Detailed information of TA system TA035357

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22890..23159
• Replicon: plasmid p18044_2
• Accession: NZ_AP022328
• Organism: Escherichia coli O25:H4 strain 18044

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECO25NV_RS25500
• Protein ID: WP_001312861.1
• Coordinates: 23001..23159 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22890..22955 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO25NV_RS25460	18176..18511	+	336	WP_077763704.1	hypothetical protein	-
ECO25NV_RS25465	18424..18630	+	207	WP_000275853.1	hypothetical protein	-
ECO25NV_RS25470	18656..19195	+	540	WP_097498141.1	single-stranded DNA-binding protein	-
ECO25NV_RS25475	19258..19492	+	235	Protein_29	DUF905 family protein	-
ECO25NV_RS25480	19558..21516	+	1959	WP_000117163.1	ParB/RepB/Spo0J family partition protein	-
ECO25NV_RS25485	21571..22005	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
ECO25NV_RS25490	22002..22721	+	720	WP_052925806.1	plasmid SOS inhibition protein A	-
ECO25NV_RS25495	22733..22921	-	189	WP_001299721.1	hypothetical protein	-
-	22733..22957	+	225	NuclAT_0	-	-
-	22733..22957	+	225	NuclAT_0	-	-
-	22733..22957	+	225	NuclAT_0	-	-
-	22733..22957	+	225	NuclAT_0	-	-
-	22890..22955	+	66	-	-	Antitoxin
ECO25NV_RS25500	23001..23159	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
ECO25NV_RS25505	23562..24212	+	651	WP_001487415.1	IS66 family insertion sequence hypothetical protein	-
ECO25NV_RS25510	24212..24559	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
ECO25NV_RS25515	24579..26150	+	1572	WP_199805184.1	IS66 family transposase	-
ECO25NV_RS25520	26768..27064	+	297	WP_001272251.1	hypothetical protein	-
ECO25NV_RS25525	27175..27996	+	822	WP_001234445.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..67915	67915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35357 WP_001312861.1 NZ_AP022328:23001-23159 [Escherichia coli O25:H4]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35357 NZ_AP022328:23001-23159 [Escherichia coli O25:H4]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT35357 NZ_AP022328:22890-22955 [Escherichia coli O25:H4]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin