Detailed information of TA system TA035350

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 101291..101533
• Replicon: plasmid p18044_1
• Accession: NZ_AP022327
• Organism: Escherichia coli O25:H4 strain 18044

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECO25NV_RS25100
• Protein ID: WP_001312861.1
• Coordinates: 101291..101449 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	101493..101533 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO25NV_RS25070	96430..97251	-	822	WP_001234469.1	DUF945 domain-containing protein	-
ECO25NV_RS25075	97370..97657	-	288	WP_000107535.1	hypothetical protein	-
ECO25NV_RS25080	97682..97888	-	207	WP_000275859.1	hypothetical protein	-
ECO25NV_RS25085	98084..98734	+	651	WP_001487415.1	IS66 family insertion sequence hypothetical protein	-
ECO25NV_RS25090	98734..99081	+	348	WP_000624618.1	IS66 family insertion sequence element accessory protein TnpB	-
ECO25NV_RS25095	99101..100672	+	1572	WP_199805184.1	IS66 family transposase	-
ECO25NV_RS25100	101291..101449	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	101493..101533	-	41	NuclAT_1	-	Antitoxin
-	101493..101533	-	41	NuclAT_1	-	Antitoxin
-	101493..101533	-	41	NuclAT_1	-	Antitoxin
-	101493..101533	-	41	NuclAT_1	-	Antitoxin
-	102977..103163	-	187	NuclAT_0	-	-
-	102977..103163	-	187	NuclAT_0	-	-
-	102977..103163	-	187	NuclAT_0	-	-
-	102977..103163	-	187	NuclAT_0	-	-
ECO25NV_RS25110	103175..103894	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
ECO25NV_RS25115	103891..104325	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
ECO25NV_RS25120	104380..106338	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA17 / aadA5 / qacE / sul1 / mph(A) / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaTEM-1B	senB	1..139450	139450
	-	inside	IScluster/Tn	-	-	98084..102929	4845

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35350 WP_001312861.1 NZ_AP022327:c101449-101291 [Escherichia coli O25:H4]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35350 NZ_AP022327:c101449-101291 [Escherichia coli O25:H4]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT35350 NZ_AP022327:c101533-101493 [Escherichia coli O25:H4]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin