Detailed information of TA system TA035345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4827907..4828509
• Replicon: chromosome
• Accession: NZ_AP022326
• Organism: Escherichia coli O25:H4 strain 18044

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: ECO25NV_RS23500
• Protein ID: WP_000897302.1
• Coordinates: 4828198..4828509 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ECO25NV_RS23495
• Protein ID: WP_000356397.1
• Coordinates: 4827907..4828197 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECO25NV_RS23465	4823214..4824143	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
ECO25NV_RS23470	4824325..4824567	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
ECO25NV_RS23475	4824857..4825705	+	849	WP_001038650.1	hypothetical protein	-
ECO25NV_RS23480	4826021..4826470	+	450	WP_032140890.1	hypothetical protein	-
ECO25NV_RS23485	4826655..4826873	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
ECO25NV_RS23490	4827270..4827548	-	279	WP_001296612.1	hypothetical protein	-
ECO25NV_RS23495	4827907..4828197	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ECO25NV_RS23500	4828198..4828509	-	312	WP_000897302.1	hypothetical protein	Toxin
ECO25NV_RS23505	4828738..4829646	+	909	WP_001385591.1	alpha/beta hydrolase	-
ECO25NV_RS23510	4829710..4830651	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ECO25NV_RS23515	4830696..4831133	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ECO25NV_RS23520	4831130..4832002	-	873	WP_000920762.1	virulence factor BrkB family protein	-
ECO25NV_RS23525	4831996..4832595	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T35345 WP_000897302.1 NZ_AP022326:c4828509-4828198 [Escherichia coli O25:H4]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T35345 NZ_AP022326:c4828509-4828198 [Escherichia coli O25:H4]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT35345 WP_000356397.1 NZ_AP022326:c4828197-4827907 [Escherichia coli O25:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT35345 NZ_AP022326:c4828197-4827907 [Escherichia coli O25:H4]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416