Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 5208346..5209139 Replicon chromosome
Accession NZ_AP022324
Organism Pseudomonas putida strain TS312

Toxin (Protein)


Gene name yhaV Uniprot ID A0A1W2CLZ8
Locus tag PPTS312_RS23605 Protein ID WP_019098849.1
Coordinates 5208669..5209139 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A1W2CMY6
Locus tag PPTS312_RS23600 Protein ID WP_019098850.1
Coordinates 5208346..5208672 (+) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PPTS312_RS23580 5203944..5205005 - 1062 WP_019098854.1 HlyD family secretion protein -
PPTS312_RS23585 5205034..5206575 - 1542 WP_161990141.1 MFS transporter -
PPTS312_RS23590 5206692..5207597 - 906 WP_019098852.1 LysR family transcriptional regulator -
PPTS312_RS23595 5207806..5208252 + 447 WP_019098851.1 universal stress protein -
PPTS312_RS23600 5208346..5208672 + 327 WP_019098850.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
PPTS312_RS23605 5208669..5209139 + 471 WP_019098849.1 type II toxin-antitoxin system YhaV family toxin Toxin
PPTS312_RS23610 5209147..5210007 - 861 WP_016713502.1 HlyD family secretion protein -
PPTS312_RS23615 5210018..5210218 - 201 WP_009685040.1 DUF1656 domain-containing protein -
PPTS312_RS23620 5210208..5212385 - 2178 WP_161990142.1 FUSC family protein -
PPTS312_RS23625 5212382..5213911 - 1530 WP_019098847.1 efflux transporter outer membrane subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(16-152)

Antitoxin

PrlF antitoxin

(8-106)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 18235.65 Da        Isoelectric Point: 9.7367

>T35321 WP_019098849.1 NZ_AP022324:5208669-5209139 [Pseudomonas putida]
MSDASRKPLVIHGWTVFAHPLFLAKLEALSTQVHAQMQKDPTGYTERNAYKRLAAIKRLAFDVIPQDPTKSEYRQGATLG
EGHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWVNDESSKRAYESHDDAYKVFQKMLDNGNPPDDWDQLLREAAQQP

Download         Length: 471 bp

>T35321 NZ_AP022324:5208669-5209139 [Pseudomonas putida]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTATTTGCCCACCCGCTGTTCCTGGCCAAACTGGA
GGCACTGAGCACTCAAGTTCATGCTCAGATGCAGAAAGACCCAACAGGTTACACGGAAAGAAATGCCTATAAGCGGCTGG
CGGCTATCAAGCGTTTGGCTTTTGATGTGATCCCTCAGGACCCGACCAAATCTGAATACCGCCAAGGCGCCACGCTGGGC
GAGGGTCACAAGCACTGGTTTCGGGCAAAGTTCTTTCAGCAGTACCGGTTGTTCTTTCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGGTCAATGACGAATCAAGCAAGCGGGCTTATGAAAGTCATGACGATGCTTACAAGGTCTTCCAGA
AAATGCTCGACAACGGCAACCCTCCCGATGATTGGGATCAGTTGCTGAGGGAAGCCGCACAGCAACCGTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12017.49 Da        Isoelectric Point: 4.3118

>AT35321 WP_019098850.1 NZ_AP022324:5208346-5208672 [Pseudomonas putida]
MATTLDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGEVILTRAEATESDPVLGKFLDFLAHDIATNPQRLQVV
DADLIARLDKLVGDAEVDLDQPLSADDE

Download         Length: 327 bp

>AT35321 NZ_AP022324:5208346-5208672 [Pseudomonas putida]
ATGGCAACCACTCTCGACGTCGAGTCCACACTGACCGATCGCTATCAGACCACTGTTCCCGAAACAGTACGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTACTCGATTCGCCCTGACGGTGAGGTGATACTCACCCGTGCAGAAGCAACCG
AGAGCGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCACACGATATCGCCACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGACCTCATCGCGCGTCTCGACAAGCTTGTCGGCGACGCAGAGGTCGACCTGGATCAACCTCTGTCGGCGGATGA
TGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W2CLZ8

Antitoxin

Source ID Structure
AlphaFold DB A0A1W2CMY6

References