Detailed information of TA system TA035281

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11247..11517
• Replicon: plasmid pBEC1-S17-ESBL-09_1
• Accession: NZ_AP022299
• Organism: Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R48_RS24515
• Protein ID: WP_001312861.1
• Coordinates: 11359..11517 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	11247..11310 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R48_RS24490	6280..7491	+	1212	WP_001390768.1	dispersin export-associated protein AatD	-
H7R48_RS24500	9929..10363	+	435	WP_139451276.1	conjugation system SOS inhibitor PsiB	-
H7R48_RS24505	10360..11079	+	720	WP_001403352.1	plasmid SOS inhibition protein A	-
-	11091..11315	+	225	NuclAT_0	-	-
-	11091..11315	+	225	NuclAT_0	-	-
-	11091..11315	+	225	NuclAT_0	-	-
-	11091..11315	+	225	NuclAT_0	-	-
H7R48_RS24510	11100..11279	-	180	WP_001309233.1	hypothetical protein	-
-	11247..11310	-	64	-	-	Antitoxin
H7R48_RS24515	11359..11517	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R48_RS24520	11820..12069	-	250	Protein_13	hypothetical protein	-
H7R48_RS24525	12370..12657	+	288	WP_000107526.1	hypothetical protein	-
H7R48_RS24530	12776..13597	+	822	WP_001234469.1	DUF945 domain-containing protein	-
H7R48_RS24535	13892..14482	-	591	WP_167575169.1	transglycosylase SLT domain-containing protein	-
H7R48_RS24540	14825..15208	+	384	WP_001151564.1	relaxosome protein TraM	-
H7R48_RS24545	15402..16088	+	687	WP_139451277.1	PAS domain-containing protein	-
H7R48_RS24550	16182..16409	+	228	WP_139451278.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	pic / agg3D / agg3C / agg3B / agg3A / aap/aspU	1..123266	123266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35281 WP_001312861.1 NZ_AP022299:11359-11517 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35281 NZ_AP022299:11359-11517 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT35281 NZ_AP022299:c11310-11247 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin