Detailed information of TA system TA035273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3950755..3951449
• Replicon: chromosome
• Accession: NZ_AP022298
• Organism: Escherichia coli strain BEC1-S17-ESBL-09

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: H7R48_RS18880
• Protein ID: WP_001521903.1
• Coordinates: 3950755..3951153 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: H7R48_RS18885
• Protein ID: WP_000554758.1
• Coordinates: 3951156..3951449 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R48_RS18850	3945924..3947168	-	1245	WP_000189541.1	esterase FrsA	-
-	3946584..3946664	-	81	NuclAT_10	-	-
-	3946584..3946664	-	81	NuclAT_10	-	-
-	3946584..3946664	-	81	NuclAT_10	-	-
-	3946584..3946664	-	81	NuclAT_10	-	-
H7R48_RS18855	3947260..3947718	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
H7R48_RS18860	3947979..3949436	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
H7R48_RS25795	3949493..3949845	-	353	Protein_3686	peptide chain release factor H	-
H7R48_RS18870	3949841..3950047	-	207	Protein_3687	RtcB family protein	-
H7R48_RS18875	3950293..3950745	-	453	WP_001059899.1	GNAT family N-acetyltransferase	-
H7R48_RS18880	3950755..3951153	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
H7R48_RS18885	3951156..3951449	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
H7R48_RS18890	3951501..3952556	-	1056	WP_001226177.1	DNA polymerase IV	-
H7R48_RS18895	3952654..3953412	-	759	WP_182924608.1	putative lateral flagellar export/assembly protein LafU	-
H7R48_RS18900	3953384..3955096	+	1713	Protein_3693	flagellar biosynthesis protein FlhA	-
H7R48_RS18905	3955535..3956032	-	498	WP_000006263.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T35273 WP_001521903.1 NZ_AP022298:c3951153-3950755 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T35273 NZ_AP022298:c3951153-3950755 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT35273 WP_000554758.1 NZ_AP022298:c3951449-3951156 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT35273 NZ_AP022298:c3951449-3951156 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3