Detailed information of TA system TA035252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4460635..4461237
• Replicon: chromosome
• Accession: NZ_AP022295
• Organism: Escherichia coli strain BEC1-S17-ESBL-07

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R29_RS21225
• Protein ID: WP_000897305.1
• Coordinates: 4460926..4461237 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R29_RS21220
• Protein ID: WP_000356397.1
• Coordinates: 4460635..4460925 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R29_RS21195	4456560..4457462	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R29_RS21200	4457459..4458094	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R29_RS21205	4458091..4459020	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
H7R29_RS21210	4459350..4459592	-	243	WP_001087409.1	hypothetical protein	-
H7R29_RS21215	4459812..4460030	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
H7R29_RS21220	4460635..4460925	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
H7R29_RS21225	4460926..4461237	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R29_RS21230	4461466..4462374	+	909	WP_001162704.1	alpha/beta hydrolase	-
H7R29_RS21235	4462438..4463379	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R29_RS21240	4463424..4463861	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
H7R29_RS21245	4463858..4464730	-	873	WP_000920762.1	virulence factor BrkB family protein	-
H7R29_RS21250	4464724..4465323	-	600	WP_001295269.1	glucose-1-phosphatase	-
H7R29_RS21255	4465422..4466207	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T35252 WP_000897305.1 NZ_AP022295:c4461237-4460926 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T35252 NZ_AP022295:c4461237-4460926 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT35252 WP_000356397.1 NZ_AP022295:c4460925-4460635 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT35252 NZ_AP022295:c4460925-4460635 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7