Detailed information of TA system TA035231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6846..7498
• Replicon: plasmid pWP9-W18-ESBL-04_1
• Accession: NZ_AP022291
• Organism: Aeromonas veronii strain WP9-W18-ESBL-04

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R81_RS22110
• Protein ID: WP_182971125.1
• Coordinates: 6846..7196 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R81_RS22115
• Protein ID: WP_042648333.1
• Coordinates: 7196..7498 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R81_RS22090	2509..2823	+	315	WP_182971123.1	MFS transporter	-
H7R81_RS22095	3036..4655	-	1620	WP_182937830.1	multicopper oxidase CueO	-
H7R81_RS22100	4899..6515	+	1617	WP_114260778.1	IS1634 family transposase	-
H7R81_RS22105	6512..6787	+	276	WP_182971124.1	hypothetical protein	-
H7R81_RS22110	6846..7196	+	351	WP_182971125.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R81_RS22115	7196..7498	+	303	WP_042648333.1	helix-turn-helix transcriptional regulator	Antitoxin
H7R81_RS22120	7682..7921	+	240	WP_182971126.1	CopG family ribbon-helix-helix protein	-
H7R81_RS22125	7911..8195	+	285	WP_182971127.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R81_RS22130	8415..8786	-	372	WP_182951776.1	hypothetical protein	-
H7R81_RS22135	8783..9109	-	327	WP_000091614.1	hypothetical protein	-
H7R81_RS22140	9133..9468	-	336	WP_000741275.1	hypothetical protein	-
H7R81_RS22145	9483..9818	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	-
H7R81_RS22150	9799..10177	-	379	Protein_14	type II toxin-antitoxin system RelE/ParE family toxin	-
H7R81_RS22155	10369..10971	+	603	WP_010465829.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..131195	131195
	-	inside	IScluster/Tn	-	-	101..13984	13883

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12964.90 Da
• Isoelectric Point: 5.7350

>T35231 WP_182971125.1 NZ_AP022291:6846-7196 [Aeromonas veronii]
MWTVKTTSLFDVWFDEQDEATQEKVLAGLLALVQGGPSVGRPLVDTIKGSRFTNLKELRVQHLGDPLRAFFAFDPLRQAI
VLCAGNKGGNDKRFYKEMLPIAEAEYAKHLTELEVG

Nucleotide

• Length: 351 bp

>T35231 NZ_AP022291:6846-7196 [Aeromonas veronii]
ATGTGGACAGTAAAAACGACCTCTTTATTCGATGTCTGGTTTGATGAACAAGACGAGGCCACGCAGGAGAAGGTTTTGGC
TGGGTTGCTTGCCTTGGTACAGGGCGGTCCATCGGTAGGGCGACCTTTAGTTGATACCATCAAGGGATCTCGCTTTACGA
ACCTCAAAGAGCTGAGAGTCCAGCACCTGGGGGATCCGCTACGAGCGTTCTTTGCTTTTGACCCGTTGCGGCAAGCCATC
GTGTTGTGTGCTGGCAATAAGGGCGGTAACGATAAGCGGTTTTACAAAGAGATGTTGCCGATCGCTGAGGCGGAATACGC
CAAGCACTTAACTGAGTTGGAGGTAGGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11165.89 Da
• Isoelectric Point: 5.1667

>AT35231 WP_042648333.1 NZ_AP022291:7196-7498 [Aeromonas veronii]
MGRTLNEMLASRSVDSQQRIQEMANELLLEVRLQALREELEVSQVELASVLGISQPAVVAMEQRGNDIKLSTMKRYVEAM
GGKLRVDVELPTGRHVGFNV

Nucleotide

• Length: 303 bp

>AT35231 NZ_AP022291:7196-7498 [Aeromonas veronii]
ATGGGAAGAACACTAAATGAGATGCTGGCCTCCCGCTCGGTGGATAGTCAGCAGCGGATCCAGGAGATGGCTAACGAGCT
GCTGCTGGAGGTGCGCTTGCAAGCGCTGCGTGAAGAGTTGGAAGTGTCCCAGGTGGAGTTGGCCTCTGTCCTTGGGATCA
GCCAACCGGCCGTTGTGGCGATGGAGCAGCGTGGGAATGATATCAAGCTATCCACCATGAAGCGTTACGTGGAAGCAATG
GGTGGGAAGCTGCGTGTTGATGTTGAGCTTCCTACAGGGAGGCATGTCGGTTTCAACGTCTAA