Detailed information of TA system TA035225

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38624..38888
• Replicon: plasmid pWP9-S17-ESBL-11_2
• Accession: NZ_AP022289
• Organism: Escherichia coli strain WP9-S17-ESBL-11

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: H7R47_RS25985
• Protein ID: WP_001331364.1
• Coordinates: 38736..38888 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	38624..38686 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R47_RS25970	34666..35736	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
H7R47_RS25975	35755..36963	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
H7R47_RS25980	37181..38167	-	987	WP_001257838.1	hypothetical protein	-
-	38328..38383	-	56	NuclAT_1	-	-
-	38328..38383	-	56	NuclAT_1	-	-
-	38328..38383	-	56	NuclAT_1	-	-
-	38328..38383	-	56	NuclAT_1	-	-
-	38624..38686	-	63	NuclAT_0	-	Antitoxin
-	38624..38686	-	63	NuclAT_0	-	Antitoxin
-	38624..38686	-	63	NuclAT_0	-	Antitoxin
-	38624..38686	-	63	NuclAT_0	-	Antitoxin
H7R47_RS25985	38736..38888	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
H7R47_RS25990	38960..39212	-	253	Protein_48	hypothetical protein	-
H7R47_RS25995	39512..39808	+	297	WP_001275298.1	hypothetical protein	-
H7R47_RS26000	39873..40049	-	177	WP_001054900.1	hypothetical protein	-
H7R47_RS26005	40381..40590	+	210	WP_182927284.1	HEAT repeat domain-containing protein	-
H7R47_RS26010	40662..41312	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R47_RS26015	41386..43554	-	2169	WP_000698357.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..82448	82448

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T35225 WP_001331364.1 NZ_AP022289:38736-38888 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T35225 NZ_AP022289:38736-38888 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT35225 NZ_AP022289:c38686-38624 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin