Detailed information of TA system TA035219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5029196..5029798
• Replicon: chromosome
• Accession: NZ_AP022287
• Organism: Escherichia coli strain WP9-S17-ESBL-11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: H7R47_RS24065
• Protein ID: WP_000897305.1
• Coordinates: 5029487..5029798 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R47_RS24060
• Protein ID: WP_000356395.1
• Coordinates: 5029196..5029486 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R47_RS24025	5024820..5025722	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
H7R47_RS24030	5025719..5026354	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
H7R47_RS24035	5026351..5027280	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
H7R47_RS24040	5027462..5027704	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
H7R47_RS24045	5027923..5028141	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
H7R47_RS24050	5028560..5028838	-	279	WP_001315112.1	hypothetical protein	-
H7R47_RS24055	5028890..5029111	-	222	WP_001550354.1	hypothetical protein	-
H7R47_RS24060	5029196..5029486	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
H7R47_RS24065	5029487..5029798	-	312	WP_000897305.1	hypothetical protein	Toxin
H7R47_RS24070	5030027..5030935	+	909	WP_001550353.1	alpha/beta hydrolase	-
H7R47_RS24075	5031103..5032917	-	1815	WP_001550352.1	hypothetical protein	-
H7R47_RS24080	5033333..5034274	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
H7R47_RS24085	5034319..5034756	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T35219 WP_000897305.1 NZ_AP022287:c5029798-5029487 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T35219 NZ_AP022287:c5029798-5029487 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT35219 WP_000356395.1 NZ_AP022287:c5029486-5029196 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT35219 NZ_AP022287:c5029486-5029196 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7