Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2050106..2050608 Replicon chromosome
Accession NZ_AP022287
Organism Escherichia coli strain WP9-S17-ESBL-11

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R47_RS09670 Protein ID WP_000767829.1
Coordinates 2050354..2050608 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R47_RS09665 Protein ID WP_001259255.1
Coordinates 2050106..2050357 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R47_RS09640 2045284..2046351 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R47_RS09645 2046351..2047421 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7R47_RS09650 2047418..2048722 - 1305 WP_039267630.1 histidinol dehydrogenase -
H7R47_RS09655 2048728..2049627 - 900 WP_000131789.1 ATP phosphoribosyltransferase -
H7R47_RS09660 2049773..2049823 - 51 WP_071593552.1 his operon leader peptide -
H7R47_RS09665 2050106..2050357 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R47_RS09670 2050354..2050608 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R47_RS09675 2050691..2051515 + 825 WP_000754737.1 SDR family oxidoreductase -
H7R47_RS09680 2051561..2052490 + 930 WP_000803351.1 LysR family transcriptional regulator -
H7R47_RS09685 2052705..2052767 + 63 WP_010723108.1 membrane protein YoeI -
H7R47_RS09690 2052757..2054115 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R47_RS09695 2054294..2055352 + 1059 WP_023568741.1 transport protein YeeE -
H7R47_RS09700 2055366..2055593 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T35198 WP_000767829.1 NZ_AP022287:2050354-2050608 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T35198 NZ_AP022287:2050354-2050608 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT35198 WP_001259255.1 NZ_AP022287:2050106-2050357 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT35198 NZ_AP022287:2050106-2050357 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References