Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 1365..1995 Replicon plasmid pWP8-W19-CRE-01_4
Accession NZ_AP022272
Organism Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)


Gene name higB Uniprot ID A0A3V9PY09
Locus tag H7R88_RS28460 Protein ID WP_001708474.1
Coordinates 1660..1995 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A704FLZ1
Locus tag H7R88_RS28455 Protein ID WP_001708475.1
Coordinates 1365..1655 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R88_RS28450 652..1305 - 654 WP_099032892.1 hypothetical protein -
H7R88_RS28455 1365..1655 - 291 WP_001708475.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
H7R88_RS28460 1660..1995 - 336 WP_001708474.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R88_RS28465 2200..2526 - 327 WP_069346703.1 hypothetical protein -
H7R88_RS28470 2839..3399 - 561 WP_117105243.1 hypothetical protein -
H7R88_RS28475 3417..3767 - 351 WP_094340935.1 hypothetical protein -
H7R88_RS28480 3802..4137 - 336 WP_094324303.1 hypothetical protein -
H7R88_RS28485 4134..4403 - 270 WP_024172976.1 hypothetical protein -
H7R88_RS28490 4417..4794 - 378 WP_148414903.1 hypothetical protein -
H7R88_RS28495 4930..6906 - 1977 WP_183114687.1 DUF87 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..41197 41197


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-104)

Antitoxin

Antitoxin MqsA/HigA-2

(21-94)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12615.30 Da        Isoelectric Point: 5.2407

>T35163 WP_001708474.1 NZ_AP022272:c1995-1660 [Raoultella ornithinolytica]
MEYYEFVETSVFTREMKTLLSDDEYKEFQTFLIENPEAGDLIVGTGGCRKVRWSRQGTGKSSGVRAIYYFYNPAGRLYML
IIYPKSEKDSLTAAEKNQLKAVVAGFKGEEG

Download         Length: 336 bp

>T35163 NZ_AP022272:c1995-1660 [Raoultella ornithinolytica]
ATGGAATACTACGAATTTGTAGAAACCTCTGTGTTCACGCGAGAGATGAAAACTCTACTCAGTGATGATGAATACAAAGA
GTTTCAAACGTTCCTCATCGAGAACCCGGAGGCGGGTGATCTGATTGTAGGAACTGGCGGTTGTCGCAAGGTTAGATGGT
CACGGCAGGGAACAGGTAAAAGTTCAGGTGTCCGTGCTATTTACTACTTCTACAACCCGGCAGGGCGCTTATACATGCTT
ATCATCTATCCAAAGAGTGAGAAGGATAGTTTGACAGCAGCAGAGAAAAACCAGCTCAAAGCGGTTGTAGCGGGTTTTAA
AGGTGAAGAGGGCTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10909.62 Da        Isoelectric Point: 10.0073

>AT35163 WP_001708475.1 NZ_AP022272:c1655-1365 [Raoultella ornithinolytica]
MRKELFDDLIASAKEAVEIHQGLREPARVTRFERPDVKAIRAKVHMKQDDFASLIGVSSSLVQSWEQGLRTPAGAALKLL
RVIERKPEFVQELREA

Download         Length: 291 bp

>AT35163 NZ_AP022272:c1655-1365 [Raoultella ornithinolytica]
ATGCGTAAAGAGCTTTTTGATGATCTGATTGCTTCTGCCAAGGAAGCTGTAGAAATCCATCAGGGTTTACGTGAGCCAGC
TCGCGTGACCCGTTTTGAACGTCCAGATGTAAAAGCTATACGGGCTAAGGTTCATATGAAACAAGATGATTTTGCCAGCC
TGATCGGGGTTAGCTCTTCTCTTGTTCAGTCATGGGAACAGGGGTTAAGAACTCCGGCAGGTGCAGCGTTAAAGCTGTTG
CGTGTCATCGAGCGTAAACCTGAATTTGTTCAGGAACTACGTGAAGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V9PY09

Antitoxin

Source ID Structure
AlphaFold DB A0A704FLZ1

References