Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 75731..76455 Replicon plasmid pWP8-W19-CRE-01_1
Accession NZ_AP022269
Organism Raoultella ornithinolytica strain WP8-W19-CRE-01

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag H7R88_RS26215 Protein ID WP_076752146.1
Coordinates 76144..76455 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H7R88_RS26210 Protein ID WP_076752145.1
Coordinates 75731..76147 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R88_RS26175 71250..71726 + 477 WP_076752132.1 transglycosylase SLT domain-containing protein -
H7R88_RS26180 71764..72294 - 531 WP_076752134.1 antirestriction protein -
H7R88_RS26185 72486..72716 + 231 WP_076752135.1 hypothetical protein -
H7R88_RS26190 72920..73753 - 834 WP_076752137.1 N-6 DNA methylase -
H7R88_RS26195 73800..74030 - 231 WP_076752138.1 hypothetical protein -
H7R88_RS26200 74119..74466 - 348 WP_076752140.1 hypothetical protein -
H7R88_RS26205 74569..74841 - 273 WP_137055850.1 hypothetical protein -
H7R88_RS26210 75731..76147 - 417 WP_076752145.1 helix-turn-helix domain-containing protein Antitoxin
H7R88_RS26215 76144..76455 - 312 WP_076752146.1 type II toxin-antitoxin system HigB family toxin Toxin
H7R88_RS26220 76605..76817 - 213 WP_076752148.1 hypothetical protein -
H7R88_RS26225 76925..77251 - 327 WP_076752150.1 hypothetical protein -
H7R88_RS26230 78110..78268 - 159 WP_076752152.1 type I toxin-antitoxin system Hok family toxin -
H7R88_RS26235 78610..79095 - 486 WP_076752153.1 GNAT family N-acetyltransferase -
H7R88_RS26240 79083..79349 - 267 WP_012540086.1 DUF1778 domain-containing protein -
H7R88_RS26245 79650..79973 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
H7R88_RS26250 79975..80688 + 714 WP_013087170.1 arsenical resistance protein ArsH -
H7R88_RS26255 80697..81242 + 546 WP_047365773.1 sigma-70 family RNA polymerase sigma factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-5 - 1..241445 241445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Antitoxin HigA

(5-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12510.31 Da        Isoelectric Point: 9.9242

>T35160 WP_076752146.1 NZ_AP022269:c76455-76144 [Raoultella ornithinolytica]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFNSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Download         Length: 312 bp

>T35160 NZ_AP022269:c76455-76144 [Raoultella ornithinolytica]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCAGCGGCCATCGATGACACATA
TCGTGTACTAAAGCGGGTTGTAGCAAAAAAACCGGATGAGCTAAAGCGATACTTTAATAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGCGGCAATAACCTCAGAATGATGTTTTTTGCTGATTTCGAGCGCGGAAAGATA
TTCGTCAAACACATTACCACCCATGCTGAATATGATCGCTTAACGAAATACTATCGGGAGCACAAAGAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15484.61 Da        Isoelectric Point: 4.4711

>AT35160 WP_076752145.1 NZ_AP022269:c76147-75731 [Raoultella ornithinolytica]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSCERSLTLDHMRALANRFQIPVSMFVD

Download         Length: 417 bp

>AT35160 NZ_AP022269:c76147-75731 [Raoultella ornithinolytica]
ATGATTTTTAGCGATGCCATTAAAGCTGCAAATGACCTTGCCAGTATTGTACCCCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCACTTAAACTCGTTGAATATCTTCTGGAACATGATCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGACAACGCGGTGGAATTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTC
TCATTGCTGCGGGTTCTTATGCAGCAACATGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTTGTGAACGCAGTCTGACCCTTGATCACATGCGAGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References