Detailed information of TA system TA035137

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47913..48177
• Replicon: plasmid pWP8-S18-ESBL-07_2
• Accession: NZ_AP022263
• Organism: Escherichia coli strain WP8-S18-ESBL-07

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: H7R25_RS24375
• Protein ID: WP_001303307.1
• Coordinates: 48025..48177 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	47913..47975 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R25_RS24360	44014..45084	-	1071	WP_000151590.1	IncI1-type conjugal transfer protein TrbB	-
H7R25_RS24365	45103..46311	-	1209	WP_001295719.1	IncI1-type conjugal transfer protein TrbA	-
-	46491..46549	-	59	NuclAT_1	-	-
-	46491..46549	-	59	NuclAT_1	-	-
-	46491..46549	-	59	NuclAT_1	-	-
-	46491..46549	-	59	NuclAT_1	-	-
H7R25_RS24370	46619..47710	-	1092	WP_001756226.1	hypothetical protein	-
-	47913..47975	-	63	NuclAT_0	-	Antitoxin
-	47913..47975	-	63	NuclAT_0	-	Antitoxin
-	47913..47975	-	63	NuclAT_0	-	Antitoxin
-	47913..47975	-	63	NuclAT_0	-	Antitoxin
H7R25_RS24375	48025..48177	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
H7R25_RS24380	48249..48500	-	252	WP_001291965.1	hypothetical protein	-
H7R25_RS24385	48800..49096	+	297	WP_001275298.1	hypothetical protein	-
H7R25_RS24390	49161..49337	-	177	WP_001054898.1	hypothetical protein	-
H7R25_RS24395	49739..49948	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
H7R25_RS24400	50020..50682	-	663	WP_000644795.1	plasmid IncI1-type surface exclusion protein ExcA	-
H7R25_RS24405	50753..52921	-	2169	WP_000698356.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaCTX-M-15	-	1..91233	91233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T35137 WP_001303307.1 NZ_AP022263:48025-48177 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T35137 NZ_AP022263:48025-48177 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT35137 NZ_AP022263:c47975-47913 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin