Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1916139..1916641 Replicon chromosome
Accession NZ_AP022261
Organism Escherichia coli strain WP8-S18-ESBL-07

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R25_RS08975 Protein ID WP_000767829.1
Coordinates 1916387..1916641 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R25_RS08970 Protein ID WP_001259255.1
Coordinates 1916139..1916390 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R25_RS08945 1911317..1912384 - 1068 WP_001544685.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R25_RS08950 1912384..1913454 - 1071 WP_000108943.1 histidinol-phosphate transaminase -
H7R25_RS08955 1913451..1914755 - 1305 WP_001544684.1 histidinol dehydrogenase -
H7R25_RS08960 1914761..1915660 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R25_RS08965 1915806..1915856 - 51 WP_001364200.1 his operon leader peptide -
H7R25_RS08970 1916139..1916390 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R25_RS08975 1916387..1916641 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R25_RS08980 1916724..1917548 + 825 WP_000754758.1 SDR family oxidoreductase -
H7R25_RS08985 1917594..1918523 + 930 WP_024192945.1 LysR family transcriptional regulator -
H7R25_RS08990 1918738..1918800 + 63 WP_010723108.1 membrane protein YoeI -
H7R25_RS08995 1918790..1920148 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T35115 WP_000767829.1 NZ_AP022261:1916387-1916641 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T35115 NZ_AP022261:1916387-1916641 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT35115 WP_001259255.1 NZ_AP022261:1916139-1916390 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT35115 NZ_AP022261:1916139-1916390 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References