Detailed information of TA system TA035091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 679217..679953
• Replicon: chromosome
• Accession: NZ_AP022256
• Organism: Klebsiella sp. WP8-S18-ESBL-06

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: H7R76_RS03315
• Protein ID: WP_106927099.1
• Coordinates: 679217..679534 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R76_RS03320
• Protein ID: WP_181358007.1
• Coordinates: 679537..679953 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R76_RS03295	674985..675953	-	969	WP_106927107.1	TerC family protein	-
H7R76_RS03300	676226..677212	-	987	WP_106927105.1	Gfo/Idh/MocA family oxidoreductase	-
H7R76_RS03305	677285..677785	-	501	WP_106927103.1	M48 family metallopeptidase	-
H7R76_RS03310	677869..679005	+	1137	WP_106927101.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
H7R76_RS03315	679217..679534	+	318	WP_106927099.1	type II toxin-antitoxin system HigB family toxin	Toxin
H7R76_RS03320	679537..679953	+	417	WP_181358007.1	helix-turn-helix domain-containing protein	Antitoxin
H7R76_RS03325	679957..681975	-	2019	WP_182966959.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
H7R76_RS03330	682113..683195	-	1083	WP_182966961.1	sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC	-
H7R76_RS03335	683192..684580	-	1389	WP_182966963.1	phosphomannomutase CpsG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-136)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12221.16 Da
• Isoelectric Point: 9.9028

>T35091 WP_106927099.1 NZ_AP022256:679217-679534 [Klebsiella sp. WP8-S18-ESBL-06]
MHLISMAAIDEGCGRFPQFAKEIKLLGKVIAKANCPTPEALRAFYPSLDNFKYLDKHYVVNIANNNIRVIALIFFESQKF
YIRHIFNHAEYDRFTDKHRTKGNKS

Nucleotide

• Length: 318 bp

>T35091 NZ_AP022256:679217-679534 [Klebsiella sp. WP8-S18-ESBL-06]
ATGCATCTGATATCAATGGCTGCAATTGACGAGGGATGTGGCAGATTTCCCCAGTTTGCGAAAGAAATTAAGCTGTTAGG
TAAGGTGATTGCGAAAGCAAACTGTCCAACGCCAGAAGCGTTGAGGGCATTTTATCCCTCATTGGATAACTTCAAATATC
TTGATAAACACTATGTTGTGAACATTGCTAACAACAATATCAGGGTGATTGCGTTGATTTTTTTCGAAAGCCAGAAGTTT
TATATCCGGCACATTTTTAACCACGCTGAGTACGATAGGTTCACAGATAAACACAGAACGAAGGGGAATAAATCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15315.90 Da
• Isoelectric Point: 5.6982

>AT35091 WP_181358007.1 NZ_AP022256:679537-679953 [Klebsiella sp. WP8-S18-ESBL-06]
MMTAKALKATKTLVAEIPLLGDNPTEADHRDALELLAELLMDDPTNPLVDLLCVRIRAYEDNQPKLRALREEIEAIPTGI
AILRTLMEQHKLTLSDFADEIGSKSMVSRVLSGQRKLTLDHVKKLAARFGISPALFID

Nucleotide

• Length: 417 bp

>AT35091 NZ_AP022256:679537-679953 [Klebsiella sp. WP8-S18-ESBL-06]
ATGATGACGGCAAAAGCCCTGAAAGCCACAAAAACGCTGGTTGCTGAAATTCCTCTTCTGGGAGATAACCCCACAGAGGC
CGATCATCGTGACGCACTGGAACTGCTGGCTGAACTGCTGATGGACGACCCAACAAATCCGTTGGTTGATCTACTGTGCG
TTCGCATCCGTGCGTATGAGGACAACCAGCCGAAATTACGTGCGTTACGTGAAGAGATTGAGGCTATTCCCACGGGCATC
GCGATTTTACGAACCCTGATGGAGCAGCATAAACTGACGCTTTCCGATTTTGCCGATGAGATTGGCAGCAAGTCGATGGT
TTCACGCGTACTCAGCGGTCAAAGAAAATTAACGCTGGATCATGTCAAAAAGCTGGCCGCTCGATTTGGCATTTCACCCG
CGTTATTTATTGATTAA