Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3696943..3697637 Replicon chromosome
Accession NZ_AP022245
Organism Escherichia coli strain WP8-S18-CRE-02

Toxin (Protein)


Gene name yafO Uniprot ID S1EXB8
Locus tag H7R30_RS18125 Protein ID WP_001263493.1
Coordinates 3696943..3697341 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag H7R30_RS18130 Protein ID WP_000554757.1
Coordinates 3697344..3697637 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R30_RS18095 3691943..3693187 - 1245 WP_000189541.1 esterase FrsA -
- 3692603..3692683 - 81 NuclAT_8 - -
- 3692603..3692683 - 81 NuclAT_8 - -
- 3692603..3692683 - 81 NuclAT_8 - -
- 3692603..3692683 - 81 NuclAT_8 - -
H7R30_RS18100 3693279..3693737 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
H7R30_RS18105 3693998..3695455 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
H7R30_RS18110 3695512..3696033 - 522 Protein_3543 peptide chain release factor H -
H7R30_RS18115 3696032..3696235 - 204 Protein_3544 RNA ligase RtcB family protein -
H7R30_RS18120 3696481..3696933 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
H7R30_RS18125 3696943..3697341 - 399 WP_001263493.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
H7R30_RS18130 3697344..3697637 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
H7R30_RS18135 3697689..3698744 - 1056 WP_001752094.1 DNA polymerase IV -
H7R30_RS18140 3698815..3699693 - 879 WP_074147741.1 putative lateral flagellar export/assembly protein LafU -
H7R30_RS18145 3699710..3700642 - 933 Protein_3550 flagellar hook-length control protein FliK -
H7R30_RS18150 3700639..3700950 - 312 WP_000414993.1 hypothetical protein -
H7R30_RS18155 3700955..3701347 - 393 WP_000285319.1 flagellar export chaperone FliS -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15472.83 Da        Isoelectric Point: 8.0949

>T35083 WP_001263493.1 NZ_AP022245:c3697341-3696943 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T35083 NZ_AP022245:c3697341-3696943 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT35083 WP_000554757.1 NZ_AP022245:c3697637-3697344 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT35083 NZ_AP022245:c3697637-3697344 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EXB8


Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References