Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1770597..1771099 Replicon chromosome
Accession NZ_AP022245
Organism Escherichia coli strain WP8-S18-CRE-02

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag H7R30_RS08420 Protein ID WP_000767829.1
Coordinates 1770845..1771099 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag H7R30_RS08415 Protein ID WP_001259255.1
Coordinates 1770597..1770848 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R30_RS08390 1765775..1766842 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
H7R30_RS08395 1766842..1767912 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
H7R30_RS08400 1767909..1769213 - 1305 WP_001593467.1 histidinol dehydrogenase -
H7R30_RS08405 1769219..1770118 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
H7R30_RS08410 1770264..1770314 - 51 WP_001364200.1 his operon leader peptide -
H7R30_RS08415 1770597..1770848 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
H7R30_RS08420 1770845..1771099 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
H7R30_RS08425 1771182..1772006 + 825 WP_000754758.1 SDR family oxidoreductase -
H7R30_RS08430 1772052..1772981 + 930 WP_000803370.1 LysR family transcriptional regulator -
H7R30_RS08435 1773196..1773258 + 63 WP_010723108.1 membrane protein YoeI -
H7R30_RS08440 1773248..1774606 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
H7R30_RS08445 1774785..1775843 + 1059 WP_152071833.1 transport protein YeeE -
H7R30_RS08450 1775857..1776084 + 228 WP_000985270.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T35071 WP_000767829.1 NZ_AP022245:1770845-1771099 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T35071 NZ_AP022245:1770845-1771099 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT35071 WP_001259255.1 NZ_AP022245:1770597-1770848 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT35071 NZ_AP022245:1770597-1770848 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References