Detailed information of TA system TA035059

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 837579..838231
• Replicon: chromosome
• Accession: NZ_AP022241
• Organism: Aeromonas caviae strain WP8-S18-CRE-01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3G9IJQ2
• Locus tag: H7R78_RS04085
• Protein ID: WP_052815688.1
• Coordinates: 837579..837929 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: H7R78_RS04090
• Protein ID: WP_052815689.1
• Coordinates: 837929..838231 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R78_RS04065	833167..834309	+	1143	WP_125117104.1	hypothetical protein	-
H7R78_RS04070	834306..835937	+	1632	WP_125117105.1	ThiF family adenylyltransferase	-
H7R78_RS04075	835925..836401	+	477	WP_125117106.1	Mov34/MPN/PAD-1 family protein	-
H7R78_RS04080	836581..837531	+	951	WP_125117107.1	DUF932 domain-containing protein	-
H7R78_RS04085	837579..837929	+	351	WP_052815688.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R78_RS04090	837929..838231	+	303	WP_052815689.1	XRE family transcriptional regulator	Antitoxin
H7R78_RS04095	838408..839400	+	993	WP_125117108.1	YqaJ viral recombinase family protein	-
H7R78_RS04100	839406..840344	+	939	WP_125117109.1	hypothetical protein	-
H7R78_RS04105	840556..841116	+	561	WP_125117110.1	lecithin retinol acyltransferase family protein	-
H7R78_RS04110	841155..841580	+	426	WP_125117111.1	DUF2787 domain-containing protein	-
H7R78_RS04115	841591..842019	+	429	WP_113721836.1	hypothetical protein	-
H7R78_RS04120	841994..842302	+	309	Protein_765	hypothetical protein	-
H7R78_RS04125	842393..842890	+	498	WP_042045083.1	DNA repair protein RadC	-
H7R78_RS04130	842887..843078	+	192	WP_077098430.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	827776..860290	32514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (5-89)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13047.95 Da
• Isoelectric Point: 6.4693

>T35059 WP_052815688.1 NZ_AP022241:837579-837929 [Aeromonas caviae]
MWTVKTTELFDAWFDVQDDATQEKVLAGLLALAQGGPSVGRPLVDTIKGSRFTNLKELRVQHQGEPLRAFFAFDPLRQAI
VLCAGNKGGNEKRFYKEMLPLAEAQYARHLAELEEK

Nucleotide

• Length: 351 bp

>T35059 NZ_AP022241:837579-837929 [Aeromonas caviae]
ATGTGGACAGTAAAAACGACCGAGCTGTTTGATGCTTGGTTTGATGTGCAAGATGACGCGACGCAGGAGAAAGTCTTGGC
CGGGCTACTCGCCTTGGCACAAGGTGGCCCATCGGTAGGGCGTCCCCTGGTCGATACCATCAAGGGCTCGCGTTTTACGA
ACTTGAAAGAGTTGAGGGTGCAGCATCAGGGGGAGCCGCTACGTGCATTCTTCGCCTTTGATCCGTTGCGACAAGCCATA
GTGCTGTGTGCTGGCAACAAGGGCGGGAACGAGAAGCGATTTTACAAAGAGATGCTGCCGTTGGCTGAGGCGCAGTACGC
TAGGCATCTGGCTGAGTTGGAGGAAAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11165.87 Da
• Isoelectric Point: 4.9062

>AT35059 WP_052815689.1 NZ_AP022241:837929-838231 [Aeromonas caviae]
MGRTLDDLLASRSEESQQRIQEMATELLLEVRIQALREELEVSQIELAKALGVSQPAVVAMEQRGSDIKLSTMKRYVEAM
GGKLRVDVELPTGRHIGFNV

Nucleotide

• Length: 303 bp

>AT35059 NZ_AP022241:837929-838231 [Aeromonas caviae]
ATGGGAAGAACACTTGACGATTTGCTGGCCTCCCGTTCAGAAGAGAGTCAGCAGCGCATCCAGGAAATGGCTACCGAGCT
GTTGCTGGAGGTGCGTATACAGGCGCTACGCGAAGAGCTGGAAGTGTCGCAGATCGAGTTGGCTAAGGCACTCGGTGTCA
GTCAGCCTGCCGTCGTGGCGATGGAGCAGCGTGGCAGTGATATCAAGCTCTCCACCATGAAACGCTATGTTGAAGCCATG
GGCGGCAAGCTGCGTGTAGATGTGGAATTGCCAACCGGACGACACATTGGGTTCAACGTCTGA

Structures

Toxin

• AlphaFold DB: A0A3G9IJQ2