Detailed information of TA system TA035054

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67841..68094
• Replicon: plasmid pWP8-S17-ESBL-12_3
• Accession: NZ_AP022225
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: H7R20_RS25465
• Protein ID: WP_001312851.1
• Coordinates: 67945..68094 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67841..67900 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS25430	63019..63480	+	462	WP_001233838.1	thermonuclease family protein	-
H7R20_RS25435	63526..63735	+	210	WP_001298565.1	hemolysin expression modulator Hha	-
H7R20_RS25440	63773..64363	+	591	WP_033807967.1	DUF2726 domain-containing protein	-
H7R20_RS25445	64550..66121	-	1572	WP_001526014.1	IS66 family transposase	-
H7R20_RS25450	66141..66488	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R20_RS25455	66488..67165	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
H7R20_RS25460	67225..67698	+	474	WP_016240489.1	hypothetical protein	-
-	67841..67900	-	60	NuclAT_1	-	Antitoxin
-	67841..67900	-	60	NuclAT_1	-	Antitoxin
-	67841..67900	-	60	NuclAT_1	-	Antitoxin
-	67841..67900	-	60	NuclAT_1	-	Antitoxin
H7R20_RS25465	67945..68094	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R20_RS25470	68379..68627	+	249	WP_000083839.1	replication regulatory protein RepA	-
H7R20_RS25475	68872..68946	+	75	WP_001365705.1	RepA leader peptide Tap	-
H7R20_RS25480	68939..69796	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70764	70764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T35054 WP_001312851.1 NZ_AP022225:67945-68094 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T35054 NZ_AP022225:67945-68094 [Escherichia coli]
ATGACGAAGTATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT35054 NZ_AP022225:c67900-67841 [Escherichia coli]
AATGTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTTGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin