Detailed information of TA system TA035050

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23492..23762
• Replicon: plasmid pWP8-S17-ESBL-12_3
• Accession: NZ_AP022225
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R20_RS25200
• Protein ID: WP_001312861.1
• Coordinates: 23604..23762 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	23492..23555 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS25170	19262..19789	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
H7R20_RS25175	19847..20080	+	234	WP_000006003.1	DUF905 family protein	-
H7R20_RS25180	20141..22105	+	1965	WP_000117316.1	ParB/RepB/Spo0J family partition protein	-
H7R20_RS25185	22174..22608	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
H7R20_RS25190	22605..23324	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	23336..23560	+	225	NuclAT_0	-	-
-	23336..23560	+	225	NuclAT_0	-	-
-	23336..23560	+	225	NuclAT_0	-	-
-	23336..23560	+	225	NuclAT_0	-	-
H7R20_RS25195	23345..23524	-	180	WP_001309233.1	hypothetical protein	-
-	23492..23555	-	64	-	-	Antitoxin
H7R20_RS25200	23604..23762	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R20_RS25205	24678..24974	+	297	WP_001272251.1	hypothetical protein	-
H7R20_RS25210	25085..25906	+	822	WP_122989966.1	DUF945 domain-containing protein	-
H7R20_RS25215	26203..26805	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
H7R20_RS25220	27125..27508	+	384	WP_001151538.1	relaxosome protein TraM	-
H7R20_RS25225	27695..28382	+	688	Protein_37	conjugal transfer transcriptional regulator TraJ	-
H7R20_RS25230	28480..28689	+	210	WP_182924585.1	TraY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70764	70764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35050 WP_001312861.1 NZ_AP022225:23604-23762 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35050 NZ_AP022225:23604-23762 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT35050 NZ_AP022225:c23555-23492 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin