Detailed information of TA system TA035045

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107414..107668
• Replicon: plasmid pWP8-S17-ESBL-12_1
• Accession: NZ_AP022223
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: H7R20_RS24335
• Protein ID: WP_015387399.1
• Coordinates: 107462..107668 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	107414..107475 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS24300	103066..103278	+	213	WP_005012601.1	hypothetical protein	-
H7R20_RS24305	103579..103668	-	90	Protein_124	IS1 family transposase	-
H7R20_RS24310	103723..104400	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
H7R20_RS24315	104400..104747	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
H7R20_RS24320	104767..106338	+	1572	WP_023149734.1	IS66 family transposase	-
H7R20_RS24325	106376..106992	-	617	Protein_128	IS1 family transposase	-
H7R20_RS24330	107093..107275	+	183	WP_000968309.1	hypothetical protein	-
-	107414..107475	-	62	NuclAT_2	-	Antitoxin
-	107414..107475	-	62	NuclAT_2	-	Antitoxin
-	107414..107475	-	62	NuclAT_2	-	Antitoxin
-	107414..107475	-	62	NuclAT_2	-	Antitoxin
H7R20_RS24335	107462..107668	+	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R20_RS24340	107952..108209	+	258	WP_000083833.1	replication regulatory protein RepA	-
H7R20_RS24345	108445..108519	+	75	WP_032336874.1	RepA leader peptide Tap	-
H7R20_RS24350	108512..108958	+	447	Protein_133	incFII family plasmid replication initiator RepA	-
H7R20_RS24355	108904..109386	-	483	Protein_134	VENN motif pre-toxin domain-containing protein	-
H7R20_RS24360	110279..111499	+	1221	WP_000410951.1	arginine deiminase	-
H7R20_RS24365	111510..112421	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / blaTEM-1B	-	1..139020	139020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T35045 WP_015387399.1 NZ_AP022223:107462-107668 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T35045 NZ_AP022223:107462-107668 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT35045 NZ_AP022223:c107475-107414 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin