Detailed information of TA system TA035041

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 67352..67592
• Replicon: plasmid pWP8-S17-ESBL-12_1
• Accession: NZ_AP022223
• Organism: Escherichia coli strain WP8-S17-ESBL-12

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: H7R20_RS24105
• Protein ID: WP_001312861.1
• Coordinates: 67434..67592 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	67352..67390 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R20_RS24060	62584..62796	+	213	WP_001348622.1	hypothetical protein	-
H7R20_RS24065	63206..63412	+	207	WP_000275856.1	hypothetical protein	-
H7R20_RS24070	63438..63977	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
H7R20_RS24075	64045..64278	+	234	WP_000005990.1	DUF905 family protein	-
H7R20_RS24080	64306..64503	+	198	Protein_79	hypothetical protein	-
H7R20_RS24085	64558..64992	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
H7R20_RS24090	64989..65708	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
H7R20_RS24095	65720..65908	-	189	WP_001299721.1	hypothetical protein	-
-	65720..65910	+	191	NuclAT_0	-	-
-	65720..65910	+	191	NuclAT_0	-	-
-	65720..65910	+	191	NuclAT_0	-	-
-	65720..65910	+	191	NuclAT_0	-	-
H7R20_RS24100	65958..67327	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	67352..67390	+	39	NuclAT_1	-	Antitoxin
-	67352..67390	+	39	NuclAT_1	-	Antitoxin
-	67352..67390	+	39	NuclAT_1	-	Antitoxin
-	67352..67390	+	39	NuclAT_1	-	Antitoxin
H7R20_RS24105	67434..67592	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
H7R20_RS24110	68513..68800	+	288	WP_000107535.1	hypothetical protein	-
H7R20_RS24115	68918..69739	+	822	WP_001234426.1	DUF945 domain-containing protein	-
H7R20_RS24120	70036..70638	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
H7R20_RS24125	70959..71342	+	384	WP_001151524.1	relaxosome protein TraM	-
H7R20_RS24130	71529..72218	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / catA1 / blaTEM-1B	-	1..139020	139020

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35041 WP_001312861.1 NZ_AP022223:67434-67592 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35041 NZ_AP022223:67434-67592 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 39 bp

>AT35041 NZ_AP022223:67352-67390 [Escherichia coli]
CATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin